Objectives: The concept change of instrumentation, its natural result is the release of smear layer and organic remaining portions that must be eliminated of the canal in order to consider it clean. Some quelantes and demineralizantes substances help the final cleaning. Methods: Thus, 12 roots in teeth were prepared following the endodontics protocol, and then, divided in 3 groups according to the final irrigation: Group I: phosphoric acid 37% in paper point, measured at the limit, for 15 seconds. Group II: EDTA- T irrigation 15 ml, for 5 minutes; Group III: citricacid 10%- irrigation 15 ml, for 5 minutes. The specimens were marked with Rhodamine B 1% and ut in the transversal section and the samples were passed through scanner and the images were recorded. The marked areas were measured according to the Image Lab software, for the dentin root canal dye penetration evaluation. Results: The data in percentages of the marked areas were determined and the results submitted to the statistics analysis for the Kruskal Wallis didn't show the differences between group II (27, 554) and group III (21, 057) however significant 0,1% when compared with the group I (33,086) Conclusions: This research concluded that all substances showed a good result in the dentin root canal cleaning.