ACTION OF DISINFECTANT SOLUTIONS ON HEALING ABUTMENT STERILIZATION PROCESS

Abstract: Introduction: Healing abutment sterilization requires a biosafety protocol for reutilization. The process can be completed with different products.Aims: To compare three disinfectant solutions used for sterilizing healing abutments.Materials and Methods: The study used five ANMAT-approved Bio fix (1) and five 3i (2) healing abutments, with diameter 4.1mm and H3mm external hexagon. Abutments were contaminated with S. mutans for 30 minutes, rinsed with PBS, seeded in SB20M broth and incubated in candle jars for 72 hours. They were removed from culture and placed in 0.2 % trypan blue solution for one hour, then rinsed again with 5ml PBS, and observed under stereoscopic microscope to count colonies. Results: CFUs were only observed on the positive controls. There was no significant difference between abutment groups or between disinfectants. Student’s test showed a value of p ≥ 0.05. Conclusion: Considering that there was no difference among the three disinfectants for either group of implants, the use of chlorhexidine is recommended because it has less corrosive action on metals. Three disinfectant solutions were used: 1) 2 % chlorhexidine solution, Certificate 008/15 M.A.S.; 2) 0.5 % sodium hypochlorite solution; 3) Surgizime L5 multienzyme detergent solution. All three were used according to the manufacturers’ instructions for 10 minutes. Each solution was used in triplicate for each abutment brand, with positive and negative controls.