IADR Abstract Archives

Bisfenol S (BPS) induces histological alterations and changes in EZRIN distribution in the rat oral mucosa

Bisphenol S (BPS) is used in a wide range of consumer products that includes thermal containers and paper products in contact with tissues and canned food containers, which acts as an endocrine disruptor due to its interaction with hormone receptors. EZRIN is an adapter protein, which stabilizes macromolecular complexes in the apical membrane of epithelial cells in polarized tissues. It was shown that EZRIN participates in the maintenance of cell polarity and is involved in cell migration and adhesion between cells and with the extracellular matrix (ECM). It also interacts with molecules involved in cell growth and cancer progression. There are studies that have shown its prognostic value in human oral cancer. Objective: In this study, we examined the effects of an environmental dose of BPS in the bucal mucosa of healthy rats and rats with polycystic ovarian syndrome (PCOS). Materials and Methods: Wistar rats, 21 days old,received a daily injection of Bisphenol S (BPS) 1 µg /kg, dehidroepiandrostenedione (DHEA) or a combination of both for studied for 20 days. Control rats were injected with vehicle, sesame oil. At the end of the protocol tissue samples were fixed in formol buffer and processed for histopathological analysis. Tissue secction were stained H&E and the expression of EZRIN was evaluated{C}[BD1]{C} . The results were statistically analyzed using the z test of difference in proportions. Results: Histopathological changes were observed in the oral mucosa of the experimental animals. Differences were found in basal epithelial hyperplasia and elongated epithelial ridges between the DHEA-BPS group and control without treatment, p<0.05. EZRIN expression was observed in the experimental group. In BPS vs control, EZRIN marker expression showed a difference of p<0.05. Conclusions: In this paper, we present the preliminary results on the effects of BPS in the oral mucosa of the rat. Epithelial alterations were observed that were accompanied by modifications in the expression of the EZRIN protein. These results suggest the need to evaluate these histopathological and EZRIN expression changes in clinical studies in patients exposed to BPS.
Argentine Division Meeting
2019 Argentine Division Meeting (Santa Fe, Argentina)
Santa Fe

Accepted Abstracts
  • Brandizzi, Daniel  ( Dpto. de Radiobiología de la Comisión Nacional de Energía Atómica (CNEA) )
  • Carino, Silvia  ( Laboratorio de Anatomía Patológica. Facultad de Odontología. Universidad Nacional de Tucumán )
  • Vargas, Carolina  ( Dpto. de Radiobiología de la Comisión Nacional de Energía Atómica (CNEA) )
  • Demacopulo, Brenda  ( Dpto. de Radiobiología de la Comisión Nacional de Energía Atómica (CNEA) )
  • Kreimann, Erica  ( Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) )
  • Comisión Nacional de Energía Atómica - CNEA
    Oral and Poster Presentations