ANALYSIS OF MICRO TOMOGRAPHIES FOR THE STUDY OF THE INTERRADICULAR BONE TISSUE OF THE 1st. LOWER MOLAR IN WISTAR RATS.
Introduction: X-ray microtomography is one of the most commonly used techniques for the study of ex vivo mineralized tissues. It uses two-dimensional X-ray images and three-dimensional reconstruction methods implementing computational algorithms, non-destructively. This equipment manages to obtain images on the micron scale. Objective: to determine the correlation of the data obtained with 2D and 3D measurements using specialized software, with respect to the interradicular bone of the 1st molar of rat. Materials and Methods: 12 Wistar rat lower hemimaxillars were used for this study. They were scanned with an X-ray microtomograph (Bruker Skyscan 1272), with the following specifications: 8.3 micron resolution, 70 Kv voltage and a current of 142 µA, 0.5 mm Al filter, 0.50 degree rotation step and rebuilt with the NRecon program (version 126.96.36.199). With the same program the microtomographs were reoriented to obtain the mediodistal view of the lower 1 molar. The new reconstructions were analyzed with the CTan analysis program (version 188.8.131.52) and thus obtain measurements of the volume and surface of mineralized (MV and MS) and non-mineralized (nMV and nMS) interradicular tissue of the lower 1st molar. The relationship MV / nMV and MS / nMS and the correlation was analyzed. The values ??obtained were statistically analyzed with the Pearson test. Results: MV / nMV and MS / nMS do not correlate in their bi-dimensional aspect, p = 0.6983 (95% CI -0.3651 to 0.5140). Conclusion: The results found that there is no correlation between the information obtained in 2D and 3D studies regarding interradicular bone, in this experimental model.