Changes in the concentration of glycogen and α - amylase in Wistar rats parotid gland by effect of 5-FU and LV calcium

Key words: Glycogen, α - amylase, cytostatics       
OBJECTIVE: Previous studies from our laboratory showed functional alterations in the activity of rat submandibular gland by the action of 5-FU and LV calcium. In order to evaluate the effect of these drugs on the functionality of the parotid gland were evaluated glycogen stores and salivary α-amylase.
METHODS: We used 20 male Wistar rats 3 months of age, body weight 300-400 g, housed in individual metabolic cages, with photoperiod of 12hrs each and divided into four experimental groups: control (C), treated with 5 - FU (20 mg / kg PC / 5 consecutive days) and treated with 5FU + LV (10 mg / kg / PC and 20 mg / kg PC / 5 consecutive days). The animals were fasted for 24 hours and sacrificed on the 7th day of the beginning of the experiment.
Glycogen levels and salivary amylase were tested immediately after the animal sacrificed at time 0 (T 0) and after 60 minutes (T 60) of incubation in glucose-free medium (KRB).
RESULTS: Glycogen level significantly decreased (T60) compared to (T 0) in all groups. In (T0), the treated groups showed a significant increase (p <0.5, p <0.01) compared to (C) Amylase glandular level, at (T 0) in (C) showed a concentration significantly higher. In (T 60) the concentration of amylase decreased significantly compared to (T 0), only in group (C). Consistent with that effect, amylase release to the incubation medium was significantly higher (p <0.5) in (C).
CONCLUSION: These results suggest the deleterious effect of (5-FU) and (LV +5- FU) on the functional activity of the parotid gland being higher with the schema (LV +5- FU).