Analysis of the different stage in amelogénesis in rats with protein undernourishment.

Badly the nutrition influences negatively in the biological processes in the different stages from the life of an individual. Nevertheless this demonstrated that when the nutritional disorder happens during the prenatal period and active stages of the growth, it causes alterations that in many cases are irreversible. The odontomorphogenesis does not escape to this situation and is for that reason that the objective of this work was to determine the effects that produce the protein undernourishment on the organ of the enamel and the volume of adamantine organic matrix produced by the ameloblasts.
Wistar rats were used, weaned to the 21 days of age and separated in two groups: control (diet lasts conventional, ad libitum) and undernourished group (devoid protein diet). The animals were sacrificed to the 35 days of experience. The jaws were dissected and they processed for previous paraffin inclusion decalcification in EDTA to 10%. Cross sections of the incisor of continuous growth at mesial level were made of 1er to molar (zone of adamantine matrix decalcified by EDTA), at level of the meeting point between 1er and 2do to molar (zone of maturation) and 2do and to 3er to molar (zone of secretion) and reddened with hematoxilina-Eosin. The cuts were observed the microscope optical and submissive histomorphométric study. They determined in each plane of cut the volume of organic matrix of the enamel, height of the ameloblasts of the intermediate layer and the starred reticulum. For the statistical analysis of the data the non-parametric test of Mann-Whitney was used. Results: the corporal weight was significantly smaller in the undernourished group at the end of the experimental period. The analyzed parameters did not show significant differences in any of the planes of cut analyzed between the groups control and undernourished. 
Conclusions: with the used degree of protein undernourishment in this study, is not possible to find alterations in the amelogénesis using cross sections of rat incisors.
Key words: amelogénesis - undernourishment - histomorphometry