IADR Abstract Archives

The Dentine Matrix Also Contains Fibromoduline and Collagen Type VI

Objective:

Collagen type-I, non-collagenous proteins and proteoglycans compose a three-dimensional network forming the dentine organic matrix. Although the presence of collagen type-I and proteoglycans such as decorin, biglycan and lumican have been extensively studied in the dentine literature, fibromodulin and collagen type-VI have been poorly characterized. The objective of this work, therefore, was to determine the presence and immonolocalization of fibromodulin and collagen type-VI in permanent healthy human dentine. 

Method:

Extracted 3rd molars (N=8) were placed in 10% formalin solution for 48h, stored in 70% (v/v) ethanol solution and demineralized with formic acid (10 vol%) for 7 days. Teeth were sectioned in 5μm slices and mounted onto glass slides. Samples were either stained with toluidine blue, for unspecific glycosaminoglycan visualization, or immunolocalized using antibodies specific for fibromodulin, biglycan, lumican, collagen type-I and -VI. Images of coronal and radicular dentine were obtained at 16x and 200x magnification for each specimen.

Result:

In low magnification, fibromodulin and collagen VI were stained in the mid-coronal regions of dentine, showing similar distribution to lumican and biglycan, despite the lower staining intensity. In contrast, toluidine blue staining was sharply concentrated in the circumpulpal region, possibly indicating the presence of chondroitin sulphate rich decorin in these locations. Collagen type I had homogenous distribution throughout the tissue. Interestingly, regions where toluidine blue staining intensity was lower matched precisely regions where PG immunostaining intensity was higher. 

Conclusion:

In summary, our results suggest that, although in lower concentration, fibromodulin and collagen VI also compose the organic dentine matrix. Furthermore, images suggest a novel and specific pattern of distribution of individual PGs and collagen type VI in human dentine.

Australian/New Zealand Division Meeting
2014 Australian/New Zealand Division Meeting (Brisbane, Australia)
Brisbane, Australia
2014

Scientific Groups
  • Kury Rodrigues, Matheus  ( Faculty of Dentistry, The University of Sydney, Sydney, , Australia )
  • Stankoska, Katerina  ( Faculty of Dentistry, The University of Sydney, Sydney, , Australia )
  • Sarram, Leila  ( Faculty of Dentistry, The University of Sydney, Sydney, , Australia )
  • Little, Chistopher  ( Kolling Institute of Medical Research, The University of Sydney, Sydney, , Australia )
  • Swain, Michael  ( Faculty of Dentistry, The University of Sydney, Sydney, , Australia )
  • Bertassoni, Luiz  ( Faculty of Dentistry, The University of Sydney, Sydney, , Australia )
  • Mineralized Tissue