IADR Abstract Archives
Biocompatibility And Mechanical Properties Of Fast-Setting Portland Cements
Objectives: Development of a new dental restorative material for primary teeth is necessary as materials currently available are technique and moisture sensitive, expensive and often exhibit questionable physical properties and longevity. This in vitro study sought to (i) determine the effects of four commercial available fast-setting Portland cements (PCs) namely - Rapid Set Concrete, Swan Cement Rapid Set Concrete, Kwikset Concrete Dry Mix, and Quick-Setting Postcrete, on cell viability, cytotoxicity and apoptosis of human dental pulp cells (hDPCs), and (ii) evaluate mechanical properties of these cements.
Methods: Cements specimens in discs (5x2mm) were fabricated from four fast-setting PCs and a glass-ionomer cement (Fuji IX GP®). The specimens were allowed to set for 24h and subsequently incubated in culture media for 3 days. hDPCs were isolated from primary teeth to determine the cell viability, cytotoxicity and apoptosis using fluorescence and luminescence-based assays. Five cylinders (12x6mm) for each cement were fabricated for compressive strength testing. After 24h and 7-days the cylindrical specimens were subjected to mechanical testing. Data were analysed using one-way analysis of variance (ANOVA) with post-hoc tests (P<0.05).
Results: Kwikset Concrete Dry Mix and Quick-setting Postcrete showed higher cell viability: cytotoxicity and cell viability: apoptosis compared to Fuji IX GP® and other fast-setting PCs. Kwikset Concrete Dry Mix and Swan Cement Rapid Set Concrete exhibited greater compressive strengths compared to Rapid Set Concrete and Quick-Setting Postcrete after 24h (P<0.05). At 7-days, Kwikset Concrete Dry Mix exhibited the greatest compressive strength compared with other PCs (33.73 ± 1.30MPa, P<0.05). There was a significant increase in compressive strength at 7-days for both Kwikset Concrete Dry Mix and Rapid Set Concrete compared to 24h (P<0.05).
Conclusions: Kwikset Concrete Dry Mix demonstrated higher biocompatibility to hDPCs and compressive strength in comparison to other commercially available fast-setting PCs; thus, making it a potential future dental restorative material for primary teeth.
Methods: Cements specimens in discs (5x2mm) were fabricated from four fast-setting PCs and a glass-ionomer cement (Fuji IX GP®). The specimens were allowed to set for 24h and subsequently incubated in culture media for 3 days. hDPCs were isolated from primary teeth to determine the cell viability, cytotoxicity and apoptosis using fluorescence and luminescence-based assays. Five cylinders (12x6mm) for each cement were fabricated for compressive strength testing. After 24h and 7-days the cylindrical specimens were subjected to mechanical testing. Data were analysed using one-way analysis of variance (ANOVA) with post-hoc tests (P<0.05).
Results: Kwikset Concrete Dry Mix and Quick-setting Postcrete showed higher cell viability: cytotoxicity and cell viability: apoptosis compared to Fuji IX GP® and other fast-setting PCs. Kwikset Concrete Dry Mix and Swan Cement Rapid Set Concrete exhibited greater compressive strengths compared to Rapid Set Concrete and Quick-Setting Postcrete after 24h (P<0.05). At 7-days, Kwikset Concrete Dry Mix exhibited the greatest compressive strength compared with other PCs (33.73 ± 1.30MPa, P<0.05). There was a significant increase in compressive strength at 7-days for both Kwikset Concrete Dry Mix and Rapid Set Concrete compared to 24h (P<0.05).
Conclusions: Kwikset Concrete Dry Mix demonstrated higher biocompatibility to hDPCs and compressive strength in comparison to other commercially available fast-setting PCs; thus, making it a potential future dental restorative material for primary teeth.