IADR Abstract Archives
3D printed scaffolds with multiple growth factors delivery system for craniofacial bone regeneration
Objectives: Craniofacial bone defects predispose functional and aesthetic complications. This project aims to develop customized functional core-shell microparticles based scaffolds for the treatment of craniofacial bone defects. The scaffolds deliver selected growth factors in a controlled manner to trigger patients’ self-repair ability and revive their innate bone regeneration.
Methods: Multiple growth factors delivery system (MDS) are developed to sequentially release cell homing factors and osteoindctive growth factors with the design of core-shell microparticles. The osteoinductive ability of the synthesized MDS with SDF-1/BMP-2 (S-B) were tested on bone marrow stromal stem cells (BMSCs). The homing effect of stem cells/progenitors into the microspheres through a chemotaxis assay with trans-wells system. 3D printing techniques are applied to fabricated customized scaffolds.
Results: For osteoinductive ability tests, the S-B group showed much higher ALP activity and more mineralized modules than MDS with BMP-2 only (B), MDS with SDF-1 only (S) or MDS (V) after 2 weeks’ culture. The RT-PCR tests of key transcription factors (Runx2, OCN, Osterix) that regulate bone development and important bone forming signals (Smad 1, 5, 8) showed much higher gene expression in S-B group. For homing effect test, S-B and S group shows an obvious chemotaxis effect on preosteoblasts by inducing more cell migration. Customized scaffolds with MDS were fabricated by 3D printing.
Conclusions: Scaffolds with multiple growth factors delivery system has an obvious cell homing and osteoinductive effects. Our 3D printed customized scaffolds provide promising platform for craniofacial bone regeneration.
Methods: Multiple growth factors delivery system (MDS) are developed to sequentially release cell homing factors and osteoindctive growth factors with the design of core-shell microparticles. The osteoinductive ability of the synthesized MDS with SDF-1/BMP-2 (S-B) were tested on bone marrow stromal stem cells (BMSCs). The homing effect of stem cells/progenitors into the microspheres through a chemotaxis assay with trans-wells system. 3D printing techniques are applied to fabricated customized scaffolds.
Results: For osteoinductive ability tests, the S-B group showed much higher ALP activity and more mineralized modules than MDS with BMP-2 only (B), MDS with SDF-1 only (S) or MDS (V) after 2 weeks’ culture. The RT-PCR tests of key transcription factors (Runx2, OCN, Osterix) that regulate bone development and important bone forming signals (Smad 1, 5, 8) showed much higher gene expression in S-B group. For homing effect test, S-B and S group shows an obvious chemotaxis effect on preosteoblasts by inducing more cell migration. Customized scaffolds with MDS were fabricated by 3D printing.
Conclusions: Scaffolds with multiple growth factors delivery system has an obvious cell homing and osteoinductive effects. Our 3D printed customized scaffolds provide promising platform for craniofacial bone regeneration.