IADR Abstract Archives
Biofilm Formation and Air-Abrasive Decontamination of Different Titanium Surfaces.
Objectives: Peri-implantitis is caused by bacterial colonisation of titanium dental implants. Although several antimicrobials and mechanical treatments have been widely used for implant decontamination, the standard of care for peri-implantitis is yet to be established. This study aims to assess in vitro the most effective air-abrasive powder to remove biofilm from bacterially contaminated titanium surfaces.
Methods: Two types of titanium discs were tested: M (Machined-surface rutile titanium) and MRS (Moderately Rough Surface). Discs were inoculated with Streptococcus sanguinis and incubated in Brain-Heart Infusion at 37° C for 72 hours to induce biofilm formation. Discs were treated with either sodium bicarbonate air-abrasive powder (SB), or water as control, for 5 seconds. Biofilm formation before and after treatment was assessed using the crystal violet assay and qualitative and quantitative SEM analyses. Changes to the surface topography of the titanium surfaces will be measured under CLSM and SEM.
Results: S. sanguinis successfully colonised both M and MRS titanium discs. Preliminary crystal violet assessment indicated that MRS discs displayed a 50% increase in biofilm compared with M discs (100 ± 1.7SD vs 49.8 ± 2.3SD). Treatment of MRS discs with either water or SB reduced the biofilm by 88% and 82% respectively (100 ± 1.7SD vs 11.8 ± 0.4SD vs 17.9 ± 1.1SD). Treatment of M discs with either water or SB reduced the biofilm by 87% and 82% respectively (100 ± 4.6SD vs 12.5 ± 0.7SD vs 18.5 ± 1.2SD).
Conclusions: Our preliminary outcomes suggest that MRS discs are more susceptible to biofilm formation than M discs, but that biofilm can be removed to a similar extent by the treatments tested to date. Further investigations utilising SEM and CLSM will compare the decontamination efficacy and surface effects of a range of powders and treatment times and provide data to standardise care for peri-implantitis.
Methods: Two types of titanium discs were tested: M (Machined-surface rutile titanium) and MRS (Moderately Rough Surface). Discs were inoculated with Streptococcus sanguinis and incubated in Brain-Heart Infusion at 37° C for 72 hours to induce biofilm formation. Discs were treated with either sodium bicarbonate air-abrasive powder (SB), or water as control, for 5 seconds. Biofilm formation before and after treatment was assessed using the crystal violet assay and qualitative and quantitative SEM analyses. Changes to the surface topography of the titanium surfaces will be measured under CLSM and SEM.
Results: S. sanguinis successfully colonised both M and MRS titanium discs. Preliminary crystal violet assessment indicated that MRS discs displayed a 50% increase in biofilm compared with M discs (100 ± 1.7SD vs 49.8 ± 2.3SD). Treatment of MRS discs with either water or SB reduced the biofilm by 88% and 82% respectively (100 ± 1.7SD vs 11.8 ± 0.4SD vs 17.9 ± 1.1SD). Treatment of M discs with either water or SB reduced the biofilm by 87% and 82% respectively (100 ± 4.6SD vs 12.5 ± 0.7SD vs 18.5 ± 1.2SD).
Conclusions: Our preliminary outcomes suggest that MRS discs are more susceptible to biofilm formation than M discs, but that biofilm can be removed to a similar extent by the treatments tested to date. Further investigations utilising SEM and CLSM will compare the decontamination efficacy and surface effects of a range of powders and treatment times and provide data to standardise care for peri-implantitis.