IADR Abstract Archives
Evaluation of laser fluorescence in human dentin following endodontic irrigation
Objectives: This study aims to evaluate bacterial laser fluorescence in uninfected and infected (E. faecalis) dentin following treatment with oxidizing agents (1% and 4% sodium hypochlorite and 3% hydrogen peroxide) using laser induced fluorescence device (DIAGNOdent Pen) over time (0 minute, 5 minutes, 10 minutes, 30 minutes, 1 hour and 24 hours).
Methods: Thirty sterilized extracted molars with unrestored anatomical crowns were used for this study. The teeth were debrided and embedded in acrylic resin and sectioned mesio-distally in 2-mm uniform thickness sections. They were then assigned to one of the two groups, infected and uninfected dentin, with sections in infected dentin group cultured with bacterial biofilm and evaluated using fluorescence microscopy. A calibrated DIAGNOdent (KaVo) hand-held device was used to test each section three times at the same spot with a custom made non-transparent jig and the readings averaged. The data were then analysed for normality and subjected to post-hoc tests.
Results: Relative to the baseline values, laser fluorescence values in uninfected and infected dentin chips subjected to treatment with oxidizing agents dropped initially and rose again after the 30-minute mark. This indicates that oxidizing agents used in modern endodontic treatment can influence laser fluoresnce detection. In other words, a true representation of bacterial presence using laser fluoresnce detection method can be negatively affected by oxidizing agents.
Conclusions: This study shows that oxidizing agents can influence to some extent the fluorescent properties of dentin. This study will hopefully assist in the development of a reliable fluorescence detection technique, ultimately facilitating bacterial removal and endodontic treatment success.
Methods: Thirty sterilized extracted molars with unrestored anatomical crowns were used for this study. The teeth were debrided and embedded in acrylic resin and sectioned mesio-distally in 2-mm uniform thickness sections. They were then assigned to one of the two groups, infected and uninfected dentin, with sections in infected dentin group cultured with bacterial biofilm and evaluated using fluorescence microscopy. A calibrated DIAGNOdent (KaVo) hand-held device was used to test each section three times at the same spot with a custom made non-transparent jig and the readings averaged. The data were then analysed for normality and subjected to post-hoc tests.
Results: Relative to the baseline values, laser fluorescence values in uninfected and infected dentin chips subjected to treatment with oxidizing agents dropped initially and rose again after the 30-minute mark. This indicates that oxidizing agents used in modern endodontic treatment can influence laser fluoresnce detection. In other words, a true representation of bacterial presence using laser fluoresnce detection method can be negatively affected by oxidizing agents.
Conclusions: This study shows that oxidizing agents can influence to some extent the fluorescent properties of dentin. This study will hopefully assist in the development of a reliable fluorescence detection technique, ultimately facilitating bacterial removal and endodontic treatment success.