IADR Abstract Archives
One abutment, one time.
Objectives: To assess the amount of contamination remaining on used HAs after sterilisation by autoclave, and to develop and compare the effectiveness of additional decontamination methods.
Methods: A total of 120 used HAs after autoclaving was divided equally into three groups. Group 1: Used HAs after autoclaving. Group 2: Used HAs after autoclaving and mechanical decontamination with air-flow polishing (EMS Master Piezon®) using erythritol powder (EMS Prophylaxis Powder). Group 3: Used HAs after autoclaving and chemical decontamination with sodium hypochlorite (NaOCl; 25g/L). Residual contaminants were stained using Phloxine B (400g/mL) and six aspects of each HA were photographed using a light microscope (2x magnification) with digital capture system (Nikon SMZ800). The proportion of stained (i.e. contaminated) area on each HA was then measured using an imaging software (ImageJ). The HAs were also examined using scanning electron microscopy (SEM) to investigate any adverse effects of each decontamination procedure on the surface characteristics of titanium HAs.
Results: The mean proportion of surface area affected by residual contaminants on the body, top (screw driver-engaging) and bottom (implant-abutment interface) surfaces for group 1 were 38.2 +/- 28.34%, 30.0 +/- 19.55% and 18.7 +/- 17.87% respectively, whereas group 2 showed 3.5 +/- 4.90%, 5.3 +/- 3.74% and 5.4 +/- 8.49% respectively; and for group 3 the results were 0.3 +/- 0.16%, 1.9 +/- 2.14% and 0.7 +/- 1.02% respectively. Autoclaving alone was insufficient for successful decontamination while additional decontamination procedures significantly reduced the proportion of residue remaining on used HAs. NaOCl was significantly more effective than air-polishing at improving decontamination on all HA surfaces. The body surface of HAs was more readily cleaned, followed by the bottom, then the top surfaces. The SEM analysis showed no detectable differences in the surface appearance of titanium HAs after additional decontamination procedures.
Conclusions: The results show that decontamination of used HAs is achievable thus strengthening the feasibility of reusing HAs.
Methods: A total of 120 used HAs after autoclaving was divided equally into three groups. Group 1: Used HAs after autoclaving. Group 2: Used HAs after autoclaving and mechanical decontamination with air-flow polishing (EMS Master Piezon®) using erythritol powder (EMS Prophylaxis Powder). Group 3: Used HAs after autoclaving and chemical decontamination with sodium hypochlorite (NaOCl; 25g/L). Residual contaminants were stained using Phloxine B (400g/mL) and six aspects of each HA were photographed using a light microscope (2x magnification) with digital capture system (Nikon SMZ800). The proportion of stained (i.e. contaminated) area on each HA was then measured using an imaging software (ImageJ). The HAs were also examined using scanning electron microscopy (SEM) to investigate any adverse effects of each decontamination procedure on the surface characteristics of titanium HAs.
Results: The mean proportion of surface area affected by residual contaminants on the body, top (screw driver-engaging) and bottom (implant-abutment interface) surfaces for group 1 were 38.2 +/- 28.34%, 30.0 +/- 19.55% and 18.7 +/- 17.87% respectively, whereas group 2 showed 3.5 +/- 4.90%, 5.3 +/- 3.74% and 5.4 +/- 8.49% respectively; and for group 3 the results were 0.3 +/- 0.16%, 1.9 +/- 2.14% and 0.7 +/- 1.02% respectively. Autoclaving alone was insufficient for successful decontamination while additional decontamination procedures significantly reduced the proportion of residue remaining on used HAs. NaOCl was significantly more effective than air-polishing at improving decontamination on all HA surfaces. The body surface of HAs was more readily cleaned, followed by the bottom, then the top surfaces. The SEM analysis showed no detectable differences in the surface appearance of titanium HAs after additional decontamination procedures.
Conclusions: The results show that decontamination of used HAs is achievable thus strengthening the feasibility of reusing HAs.