IADR Abstract Archives

An Epigenome-Wide Association Study of individuals discordant for dental caries: A Pilot Study

Objectives: To conduct a pilot study to identify potential differentially methylated regions associated with dental caries experience in a cohort of mother-child dyads.

Methods: For this pilot study, 12 mother-child dyads from South East Queensland were selected from within the Griffith University Environments for Healthy Living Birth Cohort based on low (6 dyads) and high (6 dyads) caries experience in both mother and child, measured with the International Caries Detection and Assessment System (ICDAS). Epigenetic screening was conducted on total DNA extracted from saliva using the SeqCap Epi 4M CpGIANT Enrichment Kit and HiSeq2500 Sequencer. CpG counts were analysed using the methylKit package in R. The threshold of statistical significance was set using Bonferroni correction, based on testing 435508 CpG sites (p = 1.1E10-7).

Results: Differentially methylated regions (DMR) between cases and controls were found on chromosomes 1, 2, 5, 7, 12, 20 and 22. Chromosome 12 showed the highest average methylation difference (41%) between case and control groups (p < 2.17E-103). DAVID gene function analysis of all DMR showed two clusters related to zinc metabolism and membrane protein functions.

Conclusions: This pilot EWAS identified significantly differentially methylated sites discordant for dental caries, particularly on chromosome 12, closely related to a gene promoter region responsible for cell membrane function and zinc metabolism. Further work is required to understand the mechanisms by which these epigenetically modified sites might influence susceptibility to dental caries.

IADR Australian & New Zealand Division Annual Meeting
2017 IADR Australian & New Zealand Division Annual Meeting (Adelaide, South Australia)
Adelaide, South Australia
2017

Cariology Research - Detection, Risk Assessment and Others
  • Fernando, Surani  ( Griffith University , Arundel , Queensland , Australia ;  Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia )
  • Benton, Miles  ( Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia , Brisbane , Queensland , Australia )
  • Speicher, David  ( Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia ;  Department of Laboratory Medicine, St. Joseph’s Healthcare Hamilton, Ontario, Canada , Hamilton , Ontario , Canada )
  • Bakr, Mahmoud  ( Griffith University , Arundel , Queensland , Australia ;  Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia )
  • Zhang, Ping  ( Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia )
  • Lea, Rodney  ( The Genomics Research Centre, Queensland University of Technology, Queensland, Australia , Brisbane , Queensland , Australia )
  • Scuffham, Paul  ( Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia ;  Centre for Applied Health Economics, School of Medicine, Griffith University, Queensland, Australia , Brisbane , Queensland , Australia )
  • Johnson, Newell  ( Griffith University , Arundel , Queensland , Australia ;  Menzies Health Institute Queensland, Griffith University, Queensland, Australia , Gold Coast , Queensland , Australia )
    • NONE
    Oral Session
    Translatory applications of emerging materials and techniques to study craniofacial features and anomalies 1
    Tuesday, 09/26/2017 , 01:30PM - 03:00PM