IADR Abstract Archives
Autologous platelet-derived growth factors for bone regeneration: An in-vitro study
Objectives: The aim of this study was assess the effect of five different autologous blood preparations on osteoblast activity.
Methods: Platelet rich Plasma Preparations
Using sheep whole blood, three fresh and two anticoagulant treated platelet-derived products were prepared: Advance platelet rich Fibrin (A-PRF®), Injectable platelet rich fibrin (I-PRF®), Natural clot, Leucocyte-Platelet rich plasma (L-PRP) and Pure platelet rich plasma (P-PRP) at different dilutions (20,40,60,80,100%).
Osteoblast activity:
The effect of the platelet-derived products on pH in experimental media at different time points, Osteoblast cell growth (Alamar Blue®), viability (LIVE/DEAD®), migration (Scratch® assay) and Mineralization (Alizarin Red) were determined.
Results: A-PRF had a direct and negative effect on cell viability when the concentration was 60% or higher. In contrast, its behaviour in the cell migration assay has a strong positive effect at lower concentrations and early time points. I-PRF® was shown to be the best performer in the cell growth and mineralization assays. Moreover, P-PRP an anticoagulant treated platelet-derived product, has also shown significative benefit on osteoblast behaviour compared to control. All products were demonstrated to have high pH values (alkaline) in culture compared to physiological values.
Conclusions: Autologous blood preparations were shown to be biocompatible and stimulate osteoblast growth and mineralisation suggesting this may be a useful strategy to promote bone formation in regenerative applications. The IPRF® protocol have shown enhancement on osteoblast behaviour regardless of the concentration used.
Methods: Platelet rich Plasma Preparations
Using sheep whole blood, three fresh and two anticoagulant treated platelet-derived products were prepared: Advance platelet rich Fibrin (A-PRF®), Injectable platelet rich fibrin (I-PRF®), Natural clot, Leucocyte-Platelet rich plasma (L-PRP) and Pure platelet rich plasma (P-PRP) at different dilutions (20,40,60,80,100%).
Osteoblast activity:
The effect of the platelet-derived products on pH in experimental media at different time points, Osteoblast cell growth (Alamar Blue®), viability (LIVE/DEAD®), migration (Scratch® assay) and Mineralization (Alizarin Red) were determined.
Results: A-PRF had a direct and negative effect on cell viability when the concentration was 60% or higher. In contrast, its behaviour in the cell migration assay has a strong positive effect at lower concentrations and early time points. I-PRF® was shown to be the best performer in the cell growth and mineralization assays. Moreover, P-PRP an anticoagulant treated platelet-derived product, has also shown significative benefit on osteoblast behaviour compared to control. All products were demonstrated to have high pH values (alkaline) in culture compared to physiological values.
Conclusions: Autologous blood preparations were shown to be biocompatible and stimulate osteoblast growth and mineralisation suggesting this may be a useful strategy to promote bone formation in regenerative applications. The IPRF® protocol have shown enhancement on osteoblast behaviour regardless of the concentration used.