IADR Abstract Archives
Effect of Enterococcus faecalis on osteoclastogenesis in vitro under cobalt-mimicked hypoxia
Objectives: The bone destruction in persistent apical periodontitis associated with infection and periapical hypoxia microenvironment has not been well known. We aim to investigate the effects of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia.
Methods: Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis under cobalt-mimicked hypoxia environment. The cell viability and apoptosis were detected using CCK-8 and Flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by Western blot and real-time PCR.
Results: Under cobalt-mimicked hypoxia, E. faecalis inhibited markedly the proliferation of BMMs, while E. faecalis promoted significantly apoptosis of BMMs. Differentiation of BMMs into osteoclasts was enhanced in the presence of E. faecalis under hypoxia, and the expression of Blimp, c-fos and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
Conclusions: E. faecalis markedly promoted osteoclast differentiation under cobalt-mimicked hypoxia.
Methods: Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis under cobalt-mimicked hypoxia environment. The cell viability and apoptosis were detected using CCK-8 and Flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by Western blot and real-time PCR.
Results: Under cobalt-mimicked hypoxia, E. faecalis inhibited markedly the proliferation of BMMs, while E. faecalis promoted significantly apoptosis of BMMs. Differentiation of BMMs into osteoclasts was enhanced in the presence of E. faecalis under hypoxia, and the expression of Blimp, c-fos and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
Conclusions: E. faecalis markedly promoted osteoclast differentiation under cobalt-mimicked hypoxia.