Objectives: This study compared the cytotoxicity of the bonding agents ScotchBond 1®, Prime & Bond NT® and Xeno III® on a mouse 3T3 fibroblast cell-line. Methods: The medium was exposed to the various bonding agents for 24 hours, and then transferred into well plates containing near confluent 3T3 cells. Cell viability was compared to a similar number of controls using the MTT assay. Results: All three bonding agents were found to have a cytotoxic effect on 3T3 cells. ScotchBond 1 showed a cell survival rate of 58.8%, Prime & Bond NT 62.4%, while for Xeno III only 25.3% of cells survived. No significant difference in the survival rate was found between ScotchBond 1 and Prime & Bond NT. However, the survival rate of Xeno III was significantly less than that of the other two. Furthermore, the use of a barrier of thin etched human dentine discs (~120µm) instead of artificial membranes did not show any significant difference on the toxicity of Xeno III. Conclusions: The single step self-etching bonding agent was found to be more cytotoxic (even with a thin dentine barrier) than ScotchBond 1 and Prime & Bond NT, but none of them should be used in deep cavities.
Division: Africa/Middle East Region Meeting
Meeting:2005 Africa/Middle East Region Meeting (Jabriya, Kuwait) Location: Jabriya, Kuwait
Year: 2005 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Grobler, Sias R.
( University of the Western Cape, Cape Town, N/A, South Africa
)
Olivier, A.
( University of the Western Cape, Cape Town, N/A, South Africa
)
Moodley, Ds
( University of the Western Cape, Cape Town, N/A, South Africa
)
Du Bois, A.
( University of the Western Cape, Cape Town, N/A, South Africa
)