Objectives: The purpose of the study was to compare the surfaces and subsurfaces of enamel exposed and not exposed to two different types of wine (Wine One and Wine Two) for two time periods (five and thirty minutes) with the aid of Confocal Laser Scanning Microscopy (CLSM). Methods: Twenty-eight tooth blocks were sonicated and randomly allocated to four experimental groups, each containing seven tooth-blocks and immersed in Wines One and Two. Each of the specimen teeth with the prepared enamel surfaces were individually exposed and gently agitated for 5 or 30 minutes in the 20 ml respective wines at 20°C. Description of the Erosion Grading was as follows: 0 - normal enamel; colour yellow reflected light normal enamel; Grade 1 - disruption of surface; colour greenish; smear layer gone; grooves seen; no observable pattern of enamel prisms; Grade 2 - Honeycomb patterns start emerging; Grade 3 - definite honeycomb. Results: In all the tooth specimens various grades of erosion were observed when exposed to Wines One and Two on the surface of enamel. No differences could be detected between Wines One and Two for the five-minute exposure of enamel to the wines with respect to the degree of erosion. A strong difference between the wines though was evident at the thirty-minute exposure with the greater severity occurring for Wine Two. Subsurface loss was demonstrated by means of multilayered composite displays of lesions in the body of the enamel. Erosion of Wine Two was, however, also more severe on the subsurface which was anticipated on account of the basis of selection of the two wines. Conclusion: Erosion were observed on the surface and subsurface of enamel after exposure to the wines. No erosion was seen on the control (not exposed to wines) tooth specimens. The increasing adverse erosive effect over time is clearly noted.