Acute Periapical Inflammation: Rotary Standard Profile vs. Manual Instrumentation

Objectives: The aim of this study was to histopathologically compare and evaluate the amount of acute inflammation in the periapical region caused by instrumentation of root canal system with standard Profile system, Nitiflex file/Crown-Down and K-file/Step-Back instrument/techniques, in cat's canine teeth. Methods: Four canines of 30 mature cats were randomly instrumented with the three systems and the fourth one was left intact as the negative control group (n=120). 8, 24 or 48 hours later, depending on the group the cat belonged to, vital perfusion operation with Formalin was performed. When the fixation of the tissues were complete, the teeth and their surrounding tissue were resected and sent to pathology lab for examination under light microscope. Statistical analysis was then performed using the Kruskal-Wallis test. Results: No significant difference was discovered between different instrumentation techniques, meaning that the amount of acute periapical inflammation induced by observed canal preparation techniques were alike (P>0.05). Time required for canal preparation was significantly less in standard Profile group, comparing to others (P<0.05). No instrument breakage occurred in this study. Conclusions: Though less time consuming, Standard Profile system showed no significant difference in the amount of acute inflammation of periapical region caused by preparing the canal system, when compared to manual instrumentation techniques observed in this study.