IADR Abstract Archives
Responsive Coatings Disperse Biofilms From Dental Devices
Objectives: Dental devices can restore function and confidence in individuals, but they often lead to oral complications such as tooth decay, gum disease, yeast infections and pneumonia. The aim of this study is to investigate the potential benefits of incorporating acrylated hydroxyazobenzene (AHA) in dental devices to disperse biofilms and inhibit oral infections.
Methods: High modulus formulations with methyl methacrylate were polymerized using a photo redox initiating system with benzoyl peroxide (2wt% or 4wt%) and ethyl-4-(dimethyl amino) benzoate (1wt% or 2wt%) in the presence or absence (control) of 2wt% AHA within 0.8mm x 6.5mm (thickness x diameter, V = 26.55 mm3) spacers. Sterile samples (n = 3) were incubated with 1.8×105 colony-forming-units (CFU/mL) of Candida albicans (C. albicans) for 3h at 37°C and 5% CO2 and 4×105 CFU/mL Streptococcus mutans (S. mutans), grown for 24h at 37°C and 5% CO2. Biofilm growth was quantified via CFU counts (vortex and sonication) and the zone of inhibition around the samples were measured. EliparTM (430-480 nm) was used as the light source to study biofilm dispersion. The cytocompatibility of the samples (n= 3) were studied via MTT assay using direct contact with human gingival fibroblast (HGF) cells.
Results: The AHA-samples showed at least a 12-fold reduction in C. albicans biofilm growth compared to the control samples (p < 0.05). For the S. mutans study, the AHA-samples showed 100% inhibition of bacterial growth and demonstrated a zone of inhibition (10.2 mm ± 0.30 mm), both of which were absent in the control samples. Both AHA- and control samples were deemed cytocompatibility (p > 0.05).
Conclusions: AHA samples can significantly inhibit the growth of C. albicans and S. mutans, thereby mitigating risks and complications from dental devices. The next phase of the study will focus on tailoring the transient, light-induced mechanical movement of AHA samples (photofluidization effect) from the surface of the removable dental devices dentures to enable periodic biofilm disruption from the surface of the material.
Methods: High modulus formulations with methyl methacrylate were polymerized using a photo redox initiating system with benzoyl peroxide (2wt% or 4wt%) and ethyl-4-(dimethyl amino) benzoate (1wt% or 2wt%) in the presence or absence (control) of 2wt% AHA within 0.8mm x 6.5mm (thickness x diameter, V = 26.55 mm3) spacers. Sterile samples (n = 3) were incubated with 1.8×105 colony-forming-units (CFU/mL) of Candida albicans (C. albicans) for 3h at 37°C and 5% CO2 and 4×105 CFU/mL Streptococcus mutans (S. mutans), grown for 24h at 37°C and 5% CO2. Biofilm growth was quantified via CFU counts (vortex and sonication) and the zone of inhibition around the samples were measured. EliparTM (430-480 nm) was used as the light source to study biofilm dispersion. The cytocompatibility of the samples (n= 3) were studied via MTT assay using direct contact with human gingival fibroblast (HGF) cells.
Results: The AHA-samples showed at least a 12-fold reduction in C. albicans biofilm growth compared to the control samples (p < 0.05). For the S. mutans study, the AHA-samples showed 100% inhibition of bacterial growth and demonstrated a zone of inhibition (10.2 mm ± 0.30 mm), both of which were absent in the control samples. Both AHA- and control samples were deemed cytocompatibility (p > 0.05).
Conclusions: AHA samples can significantly inhibit the growth of C. albicans and S. mutans, thereby mitigating risks and complications from dental devices. The next phase of the study will focus on tailoring the transient, light-induced mechanical movement of AHA samples (photofluidization effect) from the surface of the removable dental devices dentures to enable periodic biofilm disruption from the surface of the material.