IADR Abstract Archives
GlcNAc Enhances Streptococcus Sanguinis Probiotic Properties Against Streptococcus Mutans-Candida Albicans
Objectives: Strategies that modulate the composition and biochemical properties of the oral microbiota, fostering a health-associated microbiome, show significant efficacy in preventing and managing dental caries. Prebiotics and probiotics have been proposed for this purpose. We sought to investigate the effects of probiotic S. sanguinis BCC23 and the prebiotic N-Acetyl glucosamine (GlcNAc) against the cariogenic and synergistic pair S.mutans-C.albicans.
Methods: Biofilm formation, microbiome composition of ex vivo dental plaque biofilms and the ability to reduce caries formation in a rat model were assessed after treatment with BCC23 and GlcNAc.
Results: GlcNAc reduced C. albicans counts in single and in multi-species S. mutans-C. albicans biofilm when cocultured with S. sanguinis. This effect was consistent whether both microorganisms were inoculated together or if C. albicans biofilm was formed 24h prior to adding BCC23. GlcNAc also reduced the biomass of S. mutans and S. mutans-C. albicans in early biofilms, particularly when S. sanguinis was added after 12h of growth. Ex vivo microcosm biofilm model derived from plaque sample collected from a caries-active patient (IRB201600851) colonized with S. mutans and C. albicans revealed that the use of GlcNAc significantly reduced C. albicans abundance. Nanopore sequencing analyses showed that when the ex vivo biofilms were cultivated with S. sanguinis and GlcNAc they had increased biodiversity and beneficial streptococci were enriched. Then, we conducted a rat caries experiment using infections with S. mutans, C. albicans (or both) followed by treatments with BCC23 in the presence or absence of GlcNAc. Co-infection of S. mutans and C. albicans increased caries severity and S. mutans colonization. GlcNAc and BCC23 reduced the occurrence and severity of carious lesions and reduced S. mutans and C. albicans colonization.
Conclusions: Our results suggest that GlcNAc can be used as a prebiotic and S. sanguinis BCC23 as a probiotic to promote oral health.
Methods: Biofilm formation, microbiome composition of ex vivo dental plaque biofilms and the ability to reduce caries formation in a rat model were assessed after treatment with BCC23 and GlcNAc.
Results: GlcNAc reduced C. albicans counts in single and in multi-species S. mutans-C. albicans biofilm when cocultured with S. sanguinis. This effect was consistent whether both microorganisms were inoculated together or if C. albicans biofilm was formed 24h prior to adding BCC23. GlcNAc also reduced the biomass of S. mutans and S. mutans-C. albicans in early biofilms, particularly when S. sanguinis was added after 12h of growth. Ex vivo microcosm biofilm model derived from plaque sample collected from a caries-active patient (IRB201600851) colonized with S. mutans and C. albicans revealed that the use of GlcNAc significantly reduced C. albicans abundance. Nanopore sequencing analyses showed that when the ex vivo biofilms were cultivated with S. sanguinis and GlcNAc they had increased biodiversity and beneficial streptococci were enriched. Then, we conducted a rat caries experiment using infections with S. mutans, C. albicans (or both) followed by treatments with BCC23 in the presence or absence of GlcNAc. Co-infection of S. mutans and C. albicans increased caries severity and S. mutans colonization. GlcNAc and BCC23 reduced the occurrence and severity of carious lesions and reduced S. mutans and C. albicans colonization.
Conclusions: Our results suggest that GlcNAc can be used as a prebiotic and S. sanguinis BCC23 as a probiotic to promote oral health.