Modulating Activated Macrophage Responses With PBM Therapy for Autoimmune Skin Disease.

Objectives: One of the key markers in the development and progression of eczema is inflammation, which is primarily driven by an overactive immune response. Current predominant treatments for this condition include proactive therapies, notably steroids. This project aims to investigate photobiomodulation (PBM) as a potential therapeutic intervention, exploring its efficacy and to dissect cellular responses post treatment.
Methods: In this experiment we seeded a reporter mice macrophage (RAW-SEAP) at 25,000/well in a 48 well plate. Lipopolysaccharide from Escherichia coli (LPS E.Coli) was utilized to induce inflammatory response in cultured cells to simulate inflammation. PBM treatment was done using pulsed( 2.5, 10 and 20 Hz) and CW ( 2.5, 5,10 Mins) with near infrared(810 nm) laser at 10mW/cm^2, 3 J/cm^2, 4.5 p.J/cm^2 or 1 Einstein. TGF-��1 (2ng/ml) was used to observe its role in suppressing inflammation. Alkaline phosphatase(ALP) assay was performed at 24 and 48 hours to observe alkaline phosphatase secretion in the condition media and fluorescence was assessed with a spectrophotometer( i3Max,Molecular Devices) . All experiement was done in replica.
Results: PBM treatment demonstrated significant (n=4, p<0.05) reduction in ALP as compared to control(LPS). TGF-��1 (n=4, p<0.05) also show significant reduction in ALP compared to control (LPS). Significant changes in cellular morphology were observed between control and treatment groups.
Conclusions: The results from this study suggest PBM can be a potential non-invasive and effective treatment for inflammation. This work adds another important element to further analyze and design eczema related conditions.