IADR Abstract Archives
Impact of Light-Curing on SDF-Treated Dentin Microhardness and Silver Penetration
Objectives: To compare silver penetration depth and dentin microhardness in non-carious teeth treated with Silver Diamine Fluoride (SDF), with and without the application of light curing (LC), in vitro.
Methods: Ten non-carious human posterior permanent teeth were extracted, sterilized, and stored in artificial saliva (pH=7.0). Each tooth was mounted in a cylindrical mold, sectioned longitudinally into halves, polished, and randomly assigned to two groups (n=10): SDF-group and SDF/LC-group. Dentin Vickers microhardness was measured in three phases using a hardness-tester: the Sound Dentin (baseline after polishing), the Demineralized Dentin phase (after 24-hour immersion in demineralized solution), and the Treated Dentin phase (seven days post-application of the treatment agent to the demineralized dentin surface). The SDF-group received 38% SDF treatment, while the SDF/LC-group received 38% SDF treatment plus 20-second light curing. Specimens were embedded, sectioned into quarters, and polished for SEM analysis after hardness testing. Silver penetration depth after the Treated Dentin phase was analyzed with backscattered electron images, and the depth from the dentin surface to the deepest silver penetration was measured using ImageJ software. Data were analyzed with JMP software. A two-way ANOVA compared hardness between treatment groups and phases (α=0.05), and a one-way ANOVA compared silver penetration depth and tubule occlusion.
Results: The SDF and SDF/LC-groups showed significantly different least squares means when comparing Vickers microhardness across the three phases. However, within each phase, no significant differences were observed between the SDF-group and the SDF/LC-group, particularly in the Treated Dentin Phase, where light curing did not significantly impact Vickers hardness compared to SDF treatment alone. The mean silver penetration depth in the SDF-group was 154.5±63.8 µm, while in the SDF/LC-group was 183.5±50.1 µm.
Conclusions: The microhardness of demineralized dentin increases following the application of Silver Diamine Fluoride (SDF). However, there were no significant differences in microhardness or silver penetration between treatments using only SDF (SDF-group) and those combining SDF with light curing (SDF/LC-group).
Methods: Ten non-carious human posterior permanent teeth were extracted, sterilized, and stored in artificial saliva (pH=7.0). Each tooth was mounted in a cylindrical mold, sectioned longitudinally into halves, polished, and randomly assigned to two groups (n=10): SDF-group and SDF/LC-group. Dentin Vickers microhardness was measured in three phases using a hardness-tester: the Sound Dentin (baseline after polishing), the Demineralized Dentin phase (after 24-hour immersion in demineralized solution), and the Treated Dentin phase (seven days post-application of the treatment agent to the demineralized dentin surface). The SDF-group received 38% SDF treatment, while the SDF/LC-group received 38% SDF treatment plus 20-second light curing. Specimens were embedded, sectioned into quarters, and polished for SEM analysis after hardness testing. Silver penetration depth after the Treated Dentin phase was analyzed with backscattered electron images, and the depth from the dentin surface to the deepest silver penetration was measured using ImageJ software. Data were analyzed with JMP software. A two-way ANOVA compared hardness between treatment groups and phases (α=0.05), and a one-way ANOVA compared silver penetration depth and tubule occlusion.
Results: The SDF and SDF/LC-groups showed significantly different least squares means when comparing Vickers microhardness across the three phases. However, within each phase, no significant differences were observed between the SDF-group and the SDF/LC-group, particularly in the Treated Dentin Phase, where light curing did not significantly impact Vickers hardness compared to SDF treatment alone. The mean silver penetration depth in the SDF-group was 154.5±63.8 µm, while in the SDF/LC-group was 183.5±50.1 µm.
Conclusions: The microhardness of demineralized dentin increases following the application of Silver Diamine Fluoride (SDF). However, there were no significant differences in microhardness or silver penetration between treatments using only SDF (SDF-group) and those combining SDF with light curing (SDF/LC-group).
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