IADR Abstract Archives
Artificial Caries Study on Caries-Inhibition Effects of Experimental Candies
Objectives: To investigate the potential caries inhibition effect of experimental “healthy candies” using artificial caries model with pH cycling.
Methods: Twenty-one extracted teeth were randomly divided into 3 groups (n=7). A 3x4mm box was marked on opposite (missal and distal) sides of enamel. The remaining tooth surface was coated with nail polish. Candy solutions of sucrose control (Ring Pop lollipop) and two experimental candies (without fluoride and with fluoride, Dr. John’s Healthy Sweets, Rockford, IL) were prepared with 50g candy and 100 mL deionized water. Demineralization solution (pH= 4.25) was prepared from 2.2mM KH2PO4, 2.2mM CaCl2.2H2O, and 50mM lactic acid. All groups were undergone pH cycles between the demineralization and remineralization (candy) solutions. A total of 1000 cycles with a 3-min dwell time in each solution. Canary system was used to measure the canary number of the exposed enamel area of each tooth at 0, 500, 700 and 1000 cycles, respectively. The specimens were then embedded in epoxy resin and sectioned into 0.15mm-thick slices. Images were taken under Nikon i50 polarized light microscope. Maximum demineralized lesions depth was measured.
Results: The control group showed significantly higher Canary number (indicating caries development) after 500 pH cycles, which did not change after that. But the two experimental candies did not show significant change in Canary number except for the group without fluoride at 1000 cycles. (Figure 1A). Both candy materials showed significantly lower demineralization depth than the control (Figure 1B) with no difference between the two experimental groups.
Conclusions: Both experimental candy materials have significant effect to reduce demineralization, and therefore have potential caries-inhibition effect.
Methods: Twenty-one extracted teeth were randomly divided into 3 groups (n=7). A 3x4mm box was marked on opposite (missal and distal) sides of enamel. The remaining tooth surface was coated with nail polish. Candy solutions of sucrose control (Ring Pop lollipop) and two experimental candies (without fluoride and with fluoride, Dr. John’s Healthy Sweets, Rockford, IL) were prepared with 50g candy and 100 mL deionized water. Demineralization solution (pH= 4.25) was prepared from 2.2mM KH2PO4, 2.2mM CaCl2.2H2O, and 50mM lactic acid. All groups were undergone pH cycles between the demineralization and remineralization (candy) solutions. A total of 1000 cycles with a 3-min dwell time in each solution. Canary system was used to measure the canary number of the exposed enamel area of each tooth at 0, 500, 700 and 1000 cycles, respectively. The specimens were then embedded in epoxy resin and sectioned into 0.15mm-thick slices. Images were taken under Nikon i50 polarized light microscope. Maximum demineralized lesions depth was measured.
Results: The control group showed significantly higher Canary number (indicating caries development) after 500 pH cycles, which did not change after that. But the two experimental candies did not show significant change in Canary number except for the group without fluoride at 1000 cycles. (Figure 1A). Both candy materials showed significantly lower demineralization depth than the control (Figure 1B) with no difference between the two experimental groups.
Conclusions: Both experimental candy materials have significant effect to reduce demineralization, and therefore have potential caries-inhibition effect.
3830740_File000000.jpg