Shear Bond Strength to Enamel Following Saliva Contamination

Objectives: The purpose of this study was to evaluate the 24-hour shear bond strength (SBS) of composite resin to enamel surfaces contaminated with artificial saliva (AS) containing mucin and amylase.
Methods: Flat bonding surfaces were prepared on extracted human molars (400 grit surface). Using adhesive tape, a 4.5mm bonding window was prepared on the substrate. Groups (n=8) were prepared using the etch & rinse (ER) or self-etch (SE) modes for Prime & Bond active (PB), Scotchbond Universal Plus (SB) and AdHese Universal (AU) dental adhesives (DA). For the control (C) groups the adhesives were applied per IFU. For the saliva contaminated groups 1.0µl of AS was applied in the bonding window and passively dried at 37°C followed by application of either 5.0µl of adhesive on ground but unconditioned enamel or 3.0µl on enamel conditioned for 15 seconds with 36% phosphoric acid. After the adhesive film was air dried and light cured using an Ultradent bonding fixture Spectrum TPH3 was bonded to the prepared surfaces. After water storage for 24 hours at 37°C the specimens were debonded and SBS was calculated (MPa). 2-way ANOVA and LSD post-hoc test was used for determining group differences at p<0.05. Condition and adhesive were significant factors (p≤0.0005). 1-way ANOVA was used to identify differences per adhesive (capital letter) or condition (letter) at p<0.05.
Results: Mean and standard deviation for each test grop are reported in the table. Condition and adhesive were significant factors (p≤0.0005). 1-way ANOVA was used to identify differences per adhesive (capital letter) or condition (letter) at p<0.05.
Conclusions: Salivary contamination was a factor in the SBS of composite to enamel placed with Universal adhesives. Acid conditioned enamel generated better results. Supported by Dentsply Sirona.