IADR Abstract Archives
TEA-BASED STAINING CAUSED by MOUTH RINSES on ENAMEL
Objectives: The use of antiseptic mouth rinses in combination with coloring food diets can lead to staining. Aim of the study was to develop an in vitro test model to investigate the staining potential of mouth rinses on human enamel considering alternating intake of potentially staining beverages and toothbrushing mimicking the situation in the oral cavity.
Methods: 4 rinsing solutions were tested: octenident® (<0.1%OCT), octenident® antiseptic (0.1%OCT), Chlorhexamed® daily mouth rinse (0.06%CHX) and Chlorhexamed® Forte (0.2%CHX). Sets of 6 halved human molar crowns were initially pre-treated by soaking in artificial saliva (30min). Afterwards, the cyclic treatment was started by soaking in artificial saliva (2min), staining with black tea (1min), brushing with toothpaste, and soaking in mouth rinse (30s). Samples were rinsed after each treatment step with distilled water. Brushing was performed in a brushing simulator with reciprocating movements for 2.5s. The cycle was conducted 30 times mimicking the clinical situation of 15 days. Photographic images were taken initially, after 10, 20 and 30 cycles. Color measurements were conducted after each staining and brushing step using a VITA-Easyshade spectrophotometer for determination of ΔL. For statistical evaluation ANOVA and post-hoc Tukey test (α=0.05) were applied.
Results: ΔL-values of the enamel surfaces increased after treatment with all mouth rinses. ΔL-values showed that <0.1%OCT caused the lowest staining followed by 0.1%OCT and both CHX formulations, whereas significant differences were determined for <0.1%OCT vs. 0.2% CHX after 30 cycles (p<0.047). All other test groups did not differ significantly.
Conclusions: The developed methodology was found to be appropriate showing staining progression of mouth rinses over an application time of 15 days. The model allows to differentiate between products, i.e., <0.1%OCT showed significantly lower staining than 0.2%CHX, while staining of 0.1%OCT was comparable with both CHX products. Further studies should be performed also with more products to validate the method.
Methods: 4 rinsing solutions were tested: octenident® (<0.1%OCT), octenident® antiseptic (0.1%OCT), Chlorhexamed® daily mouth rinse (0.06%CHX) and Chlorhexamed® Forte (0.2%CHX). Sets of 6 halved human molar crowns were initially pre-treated by soaking in artificial saliva (30min). Afterwards, the cyclic treatment was started by soaking in artificial saliva (2min), staining with black tea (1min), brushing with toothpaste, and soaking in mouth rinse (30s). Samples were rinsed after each treatment step with distilled water. Brushing was performed in a brushing simulator with reciprocating movements for 2.5s. The cycle was conducted 30 times mimicking the clinical situation of 15 days. Photographic images were taken initially, after 10, 20 and 30 cycles. Color measurements were conducted after each staining and brushing step using a VITA-Easyshade spectrophotometer for determination of ΔL. For statistical evaluation ANOVA and post-hoc Tukey test (α=0.05) were applied.
Results: ΔL-values of the enamel surfaces increased after treatment with all mouth rinses. ΔL-values showed that <0.1%OCT caused the lowest staining followed by 0.1%OCT and both CHX formulations, whereas significant differences were determined for <0.1%OCT vs. 0.2% CHX after 30 cycles (p<0.047). All other test groups did not differ significantly.
Conclusions: The developed methodology was found to be appropriate showing staining progression of mouth rinses over an application time of 15 days. The model allows to differentiate between products, i.e., <0.1%OCT showed significantly lower staining than 0.2%CHX, while staining of 0.1%OCT was comparable with both CHX products. Further studies should be performed also with more products to validate the method.