Formulation and Characterization of Antibacterial Dental Bonding Agents
Objectives: To formulate an experimental antibacterial dental bonding agent and test its microtensile bond strength on dentin. Methods: Experimental self-etching monomer MAUAEP, antibacterial monomethacrylate monomers AM-II, and antibacterial dimethacrylate monomer C16DC2DMA were synthesized as previously reported. Two new experimental acidic monomers MCA and BisMEPPA were also synthesized. Experimental primer was formulated with 2%AM-II, 6.5%MAUAEP, 6.5% DEGDMA, 5% MCA, 20%HEMA, 20%EtOH, and 40%H2O. Experimental adhesive consisted of 10%C16DC2DMA, 15%BisGMA, 25%TEGDMA, 20%UEDMA, 20%HEMA and 10%BisMEPPA. The primers and adhesives of commercial antibacterial bonding agent CLEARFIL SE Protect (SE, Kurarary, Japan) and ScotchBond Multi-purpose (MP, 3M/ESPE) were used for comparison. The specimens were prepared by application and light cure of different combinations of primers and adhesives (see table 1) onto the cut dentin of extracted human teeth, building up and light curing of composite Filtek Supreme Plus (3M/ESPE), and being sectioned into bar specimens (1x1 mm cross-section). Microtensile bond strength (MTBS) of the bar specimens was tested after storage in de-ionized water for 24h and 3 months. The data were analyzed using ANOVA and Tukey test (α=0.05). Results: The results are shown in Table 1. The experimental adhesive can be used with either the experimental primer or SE primer to achieve high and long-lasting dentin bond strength. Conclusions: The experimental antibacterial dental bonding agents have similar or higher dentin bond strength and better hydrolytical stability than commercial bonding agents.