IADR Abstract Archives
Anti-Degradative Total-Etch Adhesive to Preserve Bonded Interfaces
Objectives: Investigate the effect of loading antimicrobial octenidine dihydrochloride drug-silica particles (OCT-DSPs) into a model total-etch adhesive (TE) on the physical integrity of resin-dentin interface, incubated in simulated human salivary esterase (SHSE).
Methods: Miniature Short Rod specimens were made from resin-composite bonded to dentin using a model TE or TE loaded with 10%w/w of either OCT-DSP or C-DSP (calcined particles without drug). Specimens were tested for tensile interfacial fracture toughness (FT) and fracture mode, using stereomicroscopy, immediately or after 30-days and 180-days SHSE incubation (n=10/group). At 5-day intervals, incubation media were collected and analyzed for presence of OCT and resin degradation by-product 2,2-Bis[4(2,3-hydroxypropoxy)phenyl]propane (BisHPPP) using Ultra Performance Liquid Chromatography (UPLC). In addition, degree of conversion of the adhesive was measured using Fourier transform infra-red spectroscopy (FTIR). One-way ANOVA was used for statistical analysis.
Results: OCT-DSPs or C-DSPs had no significant effect (p>0.05) on FT vs TE at all time points. All groups showed similar FT values at 0-days incubation and after 180-days incubation. There was no significant change (p>0.05) in fracture plane for all groups after 180-days incubation. Although non-significant, the fracture plane changed from resin to dentin layer after 180-days of incubation. There were no significant differences between the degree of conversion of model TE and DSP-TE. Media analysis showed significantly higher amounts (p<0.05) of OCT and BisHPPP released at the initial time points than after 90-days when a steady-state release was reached.
Conclusions: The TE adhesive groups maintained their interfacial integrity after 180-days of SHSE incubation, but degradation was continuing to take effect shown by the change in fracture plane mode and the continuous release of OCT and BisHPPP. The addition of DSPs and C-DSPs did not impact the degradation or the FT of the resin-dentin interface, suggesting that neither the particles nor OCT affected biostability.
Methods: Miniature Short Rod specimens were made from resin-composite bonded to dentin using a model TE or TE loaded with 10%w/w of either OCT-DSP or C-DSP (calcined particles without drug). Specimens were tested for tensile interfacial fracture toughness (FT) and fracture mode, using stereomicroscopy, immediately or after 30-days and 180-days SHSE incubation (n=10/group). At 5-day intervals, incubation media were collected and analyzed for presence of OCT and resin degradation by-product 2,2-Bis[4(2,3-hydroxypropoxy)phenyl]propane (BisHPPP) using Ultra Performance Liquid Chromatography (UPLC). In addition, degree of conversion of the adhesive was measured using Fourier transform infra-red spectroscopy (FTIR). One-way ANOVA was used for statistical analysis.
Results: OCT-DSPs or C-DSPs had no significant effect (p>0.05) on FT vs TE at all time points. All groups showed similar FT values at 0-days incubation and after 180-days incubation. There was no significant change (p>0.05) in fracture plane for all groups after 180-days incubation. Although non-significant, the fracture plane changed from resin to dentin layer after 180-days of incubation. There were no significant differences between the degree of conversion of model TE and DSP-TE. Media analysis showed significantly higher amounts (p<0.05) of OCT and BisHPPP released at the initial time points than after 90-days when a steady-state release was reached.
Conclusions: The TE adhesive groups maintained their interfacial integrity after 180-days of SHSE incubation, but degradation was continuing to take effect shown by the change in fracture plane mode and the continuous release of OCT and BisHPPP. The addition of DSPs and C-DSPs did not impact the degradation or the FT of the resin-dentin interface, suggesting that neither the particles nor OCT affected biostability.