IADR Abstract Archives
Magnesium Oxide Enriched Dentinogenic Effect on Human Dental Pulp Cells
Objectives: Magnesium (Mg2+) has been considered for its potential ability to accelerate proliferation and differentiation of human osteoblasts. However, to date, magnesium oxide (MgO) and its dentinogenic effects on human dental pulp cells (HDPCs) has not been investigated. OBJECTIVE: This study was designed to evaluate the stimulatory effect of different concentrations of MgO on cell attachment, proliferation and mineralization of HDPCs.
Methods: METHODS: HDPCs were cultured in six replicates with 0.5mM, 1mM, 2mM, 4mM, 8mM concentrations of supplemental MgO, 0 mM as the negative control group, lignin sulfonic acid sodium salt and xanthan gum as the vehicle control groups. Cell attachment efficiency was assessed at 16 h. Proliferation rate was evaluated at 3, 7, 10, 14 days. Both attachment efficiency and proliferation rate were assessed by crystal violet staining. Proliferation rates were normalized by the baseline of attached cells at 16 hours. Extracellular matrix mineralization was measured by Alizarin Red staining. Statistical analysis was performed using multi-way ANOVA with Wilks’ lambda test.
Results: RESULTS: Higher cell attachment efficiency was shown with 0.5mM at 16 hours compared to control groups and other concentration groups (P<0.001). Cells with 0.5mM supplemental MgO showed significantly higher proliferation rates than control groups and other concentration groups at 7, 10, and 14 days (P<0.001). The highest levels of mineralization were also observed in 0.5mM supplemental MgO at 10, and 14 days (P <0.001).
Conclusions: CONCLUSION: The optimal MgO (0.5mM) group significantly upregulated HDPCs attachment efficiency, cell proliferation, and extracellular mineralization. Magnesium oxide containing biomaterials could be a potential novel material for pulp and dentin repair in regenerative endodontics.
Methods: METHODS: HDPCs were cultured in six replicates with 0.5mM, 1mM, 2mM, 4mM, 8mM concentrations of supplemental MgO, 0 mM as the negative control group, lignin sulfonic acid sodium salt and xanthan gum as the vehicle control groups. Cell attachment efficiency was assessed at 16 h. Proliferation rate was evaluated at 3, 7, 10, 14 days. Both attachment efficiency and proliferation rate were assessed by crystal violet staining. Proliferation rates were normalized by the baseline of attached cells at 16 hours. Extracellular matrix mineralization was measured by Alizarin Red staining. Statistical analysis was performed using multi-way ANOVA with Wilks’ lambda test.
Results: RESULTS: Higher cell attachment efficiency was shown with 0.5mM at 16 hours compared to control groups and other concentration groups (P<0.001). Cells with 0.5mM supplemental MgO showed significantly higher proliferation rates than control groups and other concentration groups at 7, 10, and 14 days (P<0.001). The highest levels of mineralization were also observed in 0.5mM supplemental MgO at 10, and 14 days (P <0.001).
Conclusions: CONCLUSION: The optimal MgO (0.5mM) group significantly upregulated HDPCs attachment efficiency, cell proliferation, and extracellular mineralization. Magnesium oxide containing biomaterials could be a potential novel material for pulp and dentin repair in regenerative endodontics.