IADR Abstract Archives
A Novel Root Canal Irrigant: a Biocompatibility Evaluation Test
Objectives: To evaluate the biocompatibility of a novel root canal irrigating solution composed of [Bromelain, ethylenediaminetetraacetic acid (EDTA), and Chlorhexidine (BEC)] compared to traditional endodontic root canal irrigants.
Methods: Twenty rabbits were divided in to 4 groups according to time periods (1, 5, 14, and 21 days). A previously determined and ideal concentration of 0.1 ml of (BEC), (NaOCl), and normal saline were injected into the rabbit's dorsal subcutaneous tissue. At the end of each time period tissue around the injected site was excised for histological sections and examined by a light microscope. A quantitative analysis of inflammatory cells was performed, and data were subjected to a least significant difference test at 1% significance.
Results: Mean number of inflammatory cells was significantly less for BEC and normal silane at 1 and 5 days compared to NaOCl (p<0.001). After 14 days, (BEC), showed statistically significant lower difference (p<0.01) and at day 21 highly significant difference (p<0.001) when compared with (NaOCl). At 21 days No significant difference was noted when (BEC) experimental group was compared with the control group at p>0.01, just opposite to the (NaOCl) group which demonstrated a significantly higher mean number of inflammatory cells and tissue reaction when compared with the control group.
Conclusions: According to the findings of this study, (BEC) appears to have better biocompatibility than (NaOCl), especially at 14 and 21 days. This could imply that (BEC) can be used safely as a root canal irrigant solution.
Methods: Twenty rabbits were divided in to 4 groups according to time periods (1, 5, 14, and 21 days). A previously determined and ideal concentration of 0.1 ml of (BEC), (NaOCl), and normal saline were injected into the rabbit's dorsal subcutaneous tissue. At the end of each time period tissue around the injected site was excised for histological sections and examined by a light microscope. A quantitative analysis of inflammatory cells was performed, and data were subjected to a least significant difference test at 1% significance.
Results: Mean number of inflammatory cells was significantly less for BEC and normal silane at 1 and 5 days compared to NaOCl (p<0.001). After 14 days, (BEC), showed statistically significant lower difference (p<0.01) and at day 21 highly significant difference (p<0.001) when compared with (NaOCl). At 21 days No significant difference was noted when (BEC) experimental group was compared with the control group at p>0.01, just opposite to the (NaOCl) group which demonstrated a significantly higher mean number of inflammatory cells and tissue reaction when compared with the control group.
Conclusions: According to the findings of this study, (BEC) appears to have better biocompatibility than (NaOCl), especially at 14 and 21 days. This could imply that (BEC) can be used safely as a root canal irrigant solution.