IADR Abstract Archives
The Effect of Dietary Hesperidin on Rat vs. Mice Long Bone Homeostasis
Objectives: The objective of this study was to investigate the bone-sparing effect of dietary hesperidin (HE), one flavonoid present in citrus fruits.
Methods: In vitro, RAW cells were cultured with 1, 100, and 500 uM doses of HE for 5 days, and differentiation to osteoclasts was observed and quantified by TRAP staining. MC3T3-E1 pre-osteoblastic cells were cultured for 14, 21, and 28 days in the presence of the same concentrations of HE, and mineralization was assessed by alizarin red staining. In vivo, rodents were given dietary HE by daily oral gavage for 6 weeks (100mg/kg in Sprague-Dawley rats) and 6 and 12 weeks (500 mg/kg for C57B6 mice). Microcomputed tomography (microCT) analysis was performed.
Results: HE led to a decrease in weight of all the animals over 6-week period that was reversed over time. microCT showed that rats and mice had an increase in bone volume, trabecular thickness, and trabecular and cortical density at 6 weeks. However, at least in mice, the positive effects in bone parameters present at 6 weeks were not maintained at 12 weeks of oral gavage with daily HE. TRAP staining of RAW cells showed that HE had an anti-resorptive effect associated with higher doses however the effect on mineralization in vitro was positive at 1uM only.
Conclusions: The results of this study show a protective effect of HE on in vivo bone loss in the early HE consumption process independent of dosage, but the effect may not last as metabolic demands change. The primary effect of HE on bone homeostasis could be mostly via modulation of the resorptive activities in bone cells although low concentrations of HE could promote osteogenesis.
Methods: In vitro, RAW cells were cultured with 1, 100, and 500 uM doses of HE for 5 days, and differentiation to osteoclasts was observed and quantified by TRAP staining. MC3T3-E1 pre-osteoblastic cells were cultured for 14, 21, and 28 days in the presence of the same concentrations of HE, and mineralization was assessed by alizarin red staining. In vivo, rodents were given dietary HE by daily oral gavage for 6 weeks (100mg/kg in Sprague-Dawley rats) and 6 and 12 weeks (500 mg/kg for C57B6 mice). Microcomputed tomography (microCT) analysis was performed.
Results: HE led to a decrease in weight of all the animals over 6-week period that was reversed over time. microCT showed that rats and mice had an increase in bone volume, trabecular thickness, and trabecular and cortical density at 6 weeks. However, at least in mice, the positive effects in bone parameters present at 6 weeks were not maintained at 12 weeks of oral gavage with daily HE. TRAP staining of RAW cells showed that HE had an anti-resorptive effect associated with higher doses however the effect on mineralization in vitro was positive at 1uM only.
Conclusions: The results of this study show a protective effect of HE on in vivo bone loss in the early HE consumption process independent of dosage, but the effect may not last as metabolic demands change. The primary effect of HE on bone homeostasis could be mostly via modulation of the resorptive activities in bone cells although low concentrations of HE could promote osteogenesis.