IADR Abstract Archives
Bone ECM-mimetic 3D-printed Bioceramic Reinforced Collagen Hydrogels for Bone Regeneration
Objectives: In the current study, a reinforced heterophasic construct, which mimics natural extracellular matrix (ECM), has been developed with the use of natural sources. We tend to design ECM mimetic matrices based on 3D printed scaffold composed of β-TCP reinforced with silicon dioxide as the mineral phase filled with collagen gel encapsulating dental pulp stem cells (DPSCs) with capability of providing sustainable bioavailability of BMP2 for bone tissue engineering.
Methods: The β-TCP precursor formulations were investigated for their flow-ability for fabrication of 3D printed scaffolds. The DPSCs laden collagen hydrogel containing BMP-2 was filled into 3D-printed matrices and incubated over three weeks. The developed constructs were characterized by 3D laser scanning microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, compressive strength testing, and biological assays. The biological characterizations were including cellular adhesion, proliferation and differentiation. The osteogenesis capability of construct was eveluated by Immunofluorescence imaging and PCR analysis.
Results: The presented construct design was found to offer both mechanical features owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the collagen hydrogel. The in vitro release kinetics of the loaded BMP-2 revealed that the designed scaffolds fulfill the bioavailability of BMP-2 required for bone tissue regeneration. The in vitro characterization of scaffolds showed that the β-TCP based reinforced collagen hydrogel provide enhanced DPSCs proliferation and osteogenesis differentiation over three weeks.
Conclusions: The proposed design for the scaffold constructs can be developed for other mesenchymal stem cells and other growth factors such as vascular endothelial growth factor (VEGF) for angiogenesis induction into the scaffold construct for bone tissue engineering.
Methods: The β-TCP precursor formulations were investigated for their flow-ability for fabrication of 3D printed scaffolds. The DPSCs laden collagen hydrogel containing BMP-2 was filled into 3D-printed matrices and incubated over three weeks. The developed constructs were characterized by 3D laser scanning microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, compressive strength testing, and biological assays. The biological characterizations were including cellular adhesion, proliferation and differentiation. The osteogenesis capability of construct was eveluated by Immunofluorescence imaging and PCR analysis.
Results: The presented construct design was found to offer both mechanical features owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the collagen hydrogel. The in vitro release kinetics of the loaded BMP-2 revealed that the designed scaffolds fulfill the bioavailability of BMP-2 required for bone tissue regeneration. The in vitro characterization of scaffolds showed that the β-TCP based reinforced collagen hydrogel provide enhanced DPSCs proliferation and osteogenesis differentiation over three weeks.
Conclusions: The proposed design for the scaffold constructs can be developed for other mesenchymal stem cells and other growth factors such as vascular endothelial growth factor (VEGF) for angiogenesis induction into the scaffold construct for bone tissue engineering.
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