IADR Abstract Archives

Live 3D Printing of Osteogenic Scaffolds Into Bone Defects

Objectives: The efficacy of bone scaffold substitutes are limited by the rate of bone formation, scaffold-defect mismatch and scaffold displacement during implantation. Additive in-situ 3D printing can overcome these limitations by printing scaffolds that conform to the dimensions of the defect site. In our laboratory, we tested several nano-biosilica-based 3D scaffolds with adequate 3D printing properties to potentially improve implantability and rapid bone healing capability. It is therefore hypothesized that these scaffolds formed the intended porosity and chemistry for bone and vascular healing.
Methods: The biosilica-biopolymer scaffold was prepared by mixing Laponite with methacrylated gelatin (MAG). Sucrose was used to increase viscosity and reduce gelation of the printing ink. IRGACURE 2529 was used as a crosslinking agent. During printing, crosslinking was initiated by UV light (365nm, 40 mW/sq. cm) at the tip of printer nozzle. Scaffolds were in-situ 3D printed directly into calvaria bone defects using varied Laponite concentration (0-4 wt.%) to determine optimal bone density (microcomputed tomography) and chemical structure (x-ray absorbance spectroscopy (XAS).
Results: Scaffolds were fabricated into a mesh design with dimensions matching that of formed defects. Scaffolds absorbed blood upon in situ printing in the defect. After 4 weeks, cranial bone samples were extracted. Evaluation by micro-CT showed that nearly 55% of the bone defect was healed after 4 weeks for higher Lp- rich-MAG scaffolds vs lower Lp-containing MAG scaffolds. Empty control defects only had 11% of the defect filled with bone after 4 weeks. XAS analysis showed that the bone had a hydroxyapatite chemical structure. Histological staining confirmed the formation of oriented collagen and blood vessels within the newly formed bone.
Conclusions: 3D in situ printing of bone regenerating scaffolds improve the delivery of regenerative and reconstructive biomedical devices for the proper and rapid healing of bone fractures.
AADR/CADR Annual Meeting
2018 AADR/CADR Annual Meeting (Fort Lauderdale, Florida)
Fort Lauderdale, Florida
2018
0057
Mineralized Tissue
  • Varanasi, Venu  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • Aswath, Pranesh  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • Azimaie, Taha  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • Cebe, Tugba  ( University of Texas at Arlington , Arlington , Texas , United States )
  • Ilyas, Azhar  ( New York Institute of Technology , Old Westbury , New York , United States )
  • Ma, Chi  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • Monte, Felipe  ( University of Texas at Arlington , Arlington , Texas , United States )
  • Ahuja, Neelam  ( Texas A&M College of Dentistry , Dallas , Texas , United States )
  • Bhattacharjee, Ritesh  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • Reddy, Likith  ( Baylor College of Dentistry , Dallas , Texas , United States )
  • National Institutes of Health / National Institute for Dental and Craniofacial Research R03 (1R03DE023872)
    NONE
    Oral Session
    Keynote Address; Mineralized Tissue I
    Wednesday, 03/21/2018 , 01:30PM - 03:00PM