Immediate Antibacterial Effects After Rinsing With a Novel CPC Mouthwash

Objectives: This clinical study examined the immediate antibacterial effects of a novel dual-phase mouthwash formulated with 0.075% cetylpyridinium chloride [test] in comparison to a control
Methods: This two-cell, double-blind, randomized cross-over design clinical study enrolled 54 adults (15 males and 39 females; age range 21-68). Subjects completed a 1-week washout phase and provided an oral sample during the baseline visit for microbiological analysis. Samples for microbiological analysis were diluted and plated on enriched agar media to enumerate viable organisms after anaerobic incubation at 37°C. Subjects were randomly assigned either the test or control treatment and completed the use of treatments in the dental clinic under supervision. A post-treatment sample was collected immediately after the treatment phase and evaluated for anaerobic organisms using microbiological procedures identical to those utilized during the baseline assessment. All subjects were scheduled for a second washout phase prior to completing the study with the alternate treatment. Statistical analyses of viable organisms (transformed log₁₀) were conducted by t-test for within-treatment assessments and by analysis of covariance (ANCOVA) for between treatment comparisons.
Results: The number of viable organisms (average ± SD) at baseline for the test and control groups was 7.23±0.28 and 7.28±0.43 Log CFU/ml, respectively. At the post-treatment evaluation, the number of organisms in the test and control groups was respectively 4.01±0.57 and 7.13±0.32 Log CFU/ml and were significantly different (p<0.05). Rinsing with the test demonstrated a 99.9% reduction in oral bacteria in comparison to baseline and the control treatment.
Conclusions: Results from this clinical study demonstrate that in comparison to the control treatment, subjects rinsing with the CPC mouthwash resulted in a 99.9% reduction in oral bacteria of the mouth. Additionally, the 99.9% reductions in oral bacteria observed were also observed in comparison to the baseline.