Enzymatic Activity of Fungi Isolated From Healthy Mouths

Objectives: Determine the enzyme produced by fungi isolated from the oral cavity of healthy mouths.
Methods: The isolation was performed on potato dextrose agar medium by extension obtaining 22 isolates. The most isolated were identified as Cladosporium spp. To determine the presence of amylases, we added lugol at the Petri dishes of the colony fungi with degradation in Agar potato dextrose The activity of amylases was analyzed by zymogram. The pH and temperature at which the enzyme produced by the fungi shows the highest activity were determined.
Results: Thirteen filamentous fungi presented amylolysis activity. The activity of amylases was analyzed by zymogram and it was possible to identify that all the fungi with enzymatic activity produced α-amylase. All the fungi showed higher enzymatic activity at pH 6 and temperature close to what is normally in the mouth (40 °C).
Conclusions: In conclusion enzymatic activity may be responsible for the oral health of these people.