IADR Abstract Archives
Effect of Quercetin on Cariogenic Traits of Oral Bacteria
Objectives: Previous work demonstrated strong inhibition of Streptococcus mutans biofilm formation by quercetin in a dose dependent manner. Smokers have a higher number of caries-affected teeth than do nonsmokers, but the association among quercetin, nicotine and biofilm formation by cariogenic bacteria has not been investigated. Objective: The purpose of this study was to determine the effect of quercetin, a flavanoid found in many foods, with varying concentrations of nicotine on biofilm formation of cariogenic bacteria.
Methods: Methodology: S. mutans strains UA 159, UA 130, OMZ 175, 10499, and A32-2, Lactobacillus caseii, Actinomyces naeslundii, and Candida albicans were used in the present study. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), total absorbance, and biofilm formation of the strains treated with different concentrations of nicotine (0–32 mg/ml) with and without 125 µg/ml quercetin were investigated. Growth effects were measured in microtiter plates over a 24 hour period. Biofilm formation was measured using a crystal violet dye staining assay.
Results: Results: The MBIC and MBC of S. mutans UA 159, UA 130, OMZ 175, 10499 and the other four species with quercetin and nicotine were 16, 8, 8, 16, and 32 mg/ml and 16, 16, 16, 32 and 32 mg/ml and with nicotine only, respectively, and MIC was 0.25, 0.25, 0.25, 0.25 and 32 mg/ml, and 16, 16, 16, 32 and 32 mg/ml with and without quercetin, respectively. Biofilm of S. mutans strains UA 159, UA 130, OMZ 175, and 10499 was signficantly repressed (p<0.05) by 8.0–16.0 mg/ml of nicotine with quercetin and 16.0-32.0 mg/ml of nicotine alone.
Conclusions: Conclusion: These results suggest that quercetin represses S. mutans biofilm formation and biofilm metabolic activity without nicotine and with low concentrations of nicotine. This may provide evidence for the efficacy of quercetin in oral products for smokers.
Methods: Methodology: S. mutans strains UA 159, UA 130, OMZ 175, 10499, and A32-2, Lactobacillus caseii, Actinomyces naeslundii, and Candida albicans were used in the present study. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), total absorbance, and biofilm formation of the strains treated with different concentrations of nicotine (0–32 mg/ml) with and without 125 µg/ml quercetin were investigated. Growth effects were measured in microtiter plates over a 24 hour period. Biofilm formation was measured using a crystal violet dye staining assay.
Results: Results: The MBIC and MBC of S. mutans UA 159, UA 130, OMZ 175, 10499 and the other four species with quercetin and nicotine were 16, 8, 8, 16, and 32 mg/ml and 16, 16, 16, 32 and 32 mg/ml and with nicotine only, respectively, and MIC was 0.25, 0.25, 0.25, 0.25 and 32 mg/ml, and 16, 16, 16, 32 and 32 mg/ml with and without quercetin, respectively. Biofilm of S. mutans strains UA 159, UA 130, OMZ 175, and 10499 was signficantly repressed (p<0.05) by 8.0–16.0 mg/ml of nicotine with quercetin and 16.0-32.0 mg/ml of nicotine alone.
Conclusions: Conclusion: These results suggest that quercetin represses S. mutans biofilm formation and biofilm metabolic activity without nicotine and with low concentrations of nicotine. This may provide evidence for the efficacy of quercetin in oral products for smokers.