Effect of Different Mouthrinses on Titanium Surface and Chemical Composition

Objectives: To determine the effect of application time of two mouthrinses on grade 4 titanium (with and without sandblasting treatment) surface and chemical composition.
Methods: In-vitro Experimental study. Protocol was approved by the IRB. 30 grade 4 titanium disks, were divided into 3 groups: a control group in where 10 titanium disks were immersed in artificial saliva (G1), an experimental group 2, in where other 10 titanium disks were immersed in a mouthrinse without fluoride “PROHealth, Oral-B” (G2) and other experimental group 3 in where the remaining 10 disks were immersed in mouthrinse with fluoride “COMPLETE, Oral-B” (G3). Half of the surface of each disk was sandblasted and the other no. Aging through storage was conducted into artificial saliva for 20 days at 37 degrees Celcius. All the specimens were then submerged in their respective mouthrinses, and the control group was kept in artificial saliva. Chemical composition analysis was conducted using spectrometry in order to determinate differences within and between the groups. Surface analysis was performed using a Scanning Electron Microscopy (SEM) JEOL- JSM 6490-LV. All measurements were performed once a month during three months. Statistical analysis was performed using one-way ANOVA, Chi-square, Shapiro Wilk, Levene, Kruskal Wallis, Grubss and Fisher tests.
Results: Statistical significant changes were observed when control group G1 was compared with G2 and G3, in where a decrease in the porosity of the surface through the time was present. No statistical significant differences were observed when chemical composition through the time, between the control group and groups G2 and G3 was compared.
Conclusions: Mouthrinses with and without fluoride induce titanium passivation through the time. Mouthrinses without fluoride did not significantly influence surface changes of the titanium disks, however a larger presence of porosities was found during the first month.