Fluoride Update From F-varnish or GIC by Extracted Teeth
Objectives: The prevalence of cervical lesions (CL) is increasing because teeth are being retained longer. Often CL are associated with root canal treatments (RCT). Our goal was understand the ability of F-varnish (5%NaF) and Glass Ionomer Cement (GIC) to deposit fluoride within naturally created CLs of extracted teeth as a model of RCT before and after acid extractions. Methods: 6 teeth with CLs were sectioned vertically to yield matched pairs of samples A and B. The A-samples were extracted 9-times with HClO4 until the extracted-[F] did not change. Both A and B-samples received a 5%NaF varnish for 3h in DI-H2O, F was extracted 4 times. Subsequently, both A and B-samples received GIC for 7-d in DI-H2O, F was extracted 4 times. Results: Initial 9 extractions for A-samples were higher [F] on the outermost layers (0.46±0.53)ppmF compared to the innermost extraction layer (0.11±0.06)ppmF. Lower [F] were found in each extraction until the 5th when the [F] became a constant baseline value. [F] after the application of the F-varnish was higher (p<0.03) than baseline for both A and B-samples. After application of GIC the [F] over 4 sequential extractions was significantly higher for both A and B-samples than at baseline (p<0.001). A-samples (0.44±0.27 to 0.31±0.21)ppmF were higher than the un-extracted B-samples (0.41±0.11 to 0.20±0.10)ppmF. Conclusions: The accumulated [F] on the surfaces of CL from the environmental history of a tooth is easily removed with 3 or 4 acid extractions. Application of 5%NaF-varnish on the CL increases the surface [F]. Application of GIC increases both the surface [F] and depth of penetration into the root tissue. The CL surface of a extracted tooth can take up F from either F-varnish or GIC. This suggests that the CL of an RCT tooth may benefit from topical fluoride treatment.