IADR Abstract Archives
Antimicrobial Potential of Resin Matrices With Coffee Compounds
Objectives: The aim was to synthesize experimental resin matrices loaded with 3 potentially bioactive compounds of coffee: trigonelline (TR), chlorogenic acid (CA) and nicotinic acid (NA), and correlate the release of the compound with its antimicrobial activity.
Methods: The minimum inhibitory concentration (MIC) against Streptococcus mutans was determined using the wild-type strain UA159 (24h of incubation at 37°C under 5% carbon dioxide). The compounds were then incorporated into resin matrices composed of Bis-GMA/TEGDMA (70/30% w/w) and camphorquinone/ethyl-4-dimethylaminobenzoate (0.5/1% w/w). Discs (10mmx1mm) were light cured for 40 sec (~550 mW/cm2), stored in 2ml of deionized water for 24 hours, and the released compounds measured by HPLC with a C18 column. For the evaluation of antimicrobial activity, disks (6mmx2mm) of each compound and a control group without antimicrobial agent (cured as above) were incubated with S. mutans to measure its biofilm formation on the disks (24h of incubation at 37°C under 5% CO2). The antimicrobial activity was analyzed by luciferase assay (GloMax® Discover System) to quantitate viable cells adhered to the disk surfaces. One way ANOVA/Tukey’s test were used to compare the groups (α = 0.05).
Results: The MIC for the compounds were: TR = 6mg/ml, CA = 6mg/ml, NA = 4mg/ml. The results demonstrated that trigonelline had a higher release, reaching a concentration very close to the MIC value: TR = 5.9±0.6mg/ml, CA = 2.9±0.01mg/ml, NA = 2.9±0.1mg/ml. Chlorogenic acid and nicotine were released at levels below their MIC. Luciferase assays demonstrated significant difference between groups (Control>CA>NA>TR). Trigonelline had a potent anti-biofilm capacity that is more effective against S. mutans than the other compounds (Figure).
Conclusions: All three compounds tested showed antimicrobial activity, which make them useful for consideration as antimicrobial additives for dental resins.
Methods: The minimum inhibitory concentration (MIC) against Streptococcus mutans was determined using the wild-type strain UA159 (24h of incubation at 37°C under 5% carbon dioxide). The compounds were then incorporated into resin matrices composed of Bis-GMA/TEGDMA (70/30% w/w) and camphorquinone/ethyl-4-dimethylaminobenzoate (0.5/1% w/w). Discs (10mmx1mm) were light cured for 40 sec (~550 mW/cm2), stored in 2ml of deionized water for 24 hours, and the released compounds measured by HPLC with a C18 column. For the evaluation of antimicrobial activity, disks (6mmx2mm) of each compound and a control group without antimicrobial agent (cured as above) were incubated with S. mutans to measure its biofilm formation on the disks (24h of incubation at 37°C under 5% CO2). The antimicrobial activity was analyzed by luciferase assay (GloMax® Discover System) to quantitate viable cells adhered to the disk surfaces. One way ANOVA/Tukey’s test were used to compare the groups (α = 0.05).
Results: The MIC for the compounds were: TR = 6mg/ml, CA = 6mg/ml, NA = 4mg/ml. The results demonstrated that trigonelline had a higher release, reaching a concentration very close to the MIC value: TR = 5.9±0.6mg/ml, CA = 2.9±0.01mg/ml, NA = 2.9±0.1mg/ml. Chlorogenic acid and nicotine were released at levels below their MIC. Luciferase assays demonstrated significant difference between groups (Control>CA>NA>TR). Trigonelline had a potent anti-biofilm capacity that is more effective against S. mutans than the other compounds (Figure).
Conclusions: All three compounds tested showed antimicrobial activity, which make them useful for consideration as antimicrobial additives for dental resins.
IMAGES
2860633_File000000.jpg
2860633_File000000.jpg