Lipopolysaccharide Induced Non-coding RNA Response in Macrophage and Fibroblast Cells

Objectives: Our goal is to examine in vitro the effect of LPS on the expression of ncRNAs by macrophages and fibroblasts. Gingival fibroblasts and macrophages function as immune-regulatory cells to choreograph the innate immune response to oral pathogenic challenges. Expression of bacterial products, including lipopolysaccharide (LPS) and gingipains, modulate the host immune response to affect a favorable microenvironment for pathogen colonization. Non-coding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), have gained traction as critical regulators of immune function. Understanding the role of ncRNAs in the oral cavity immune response to bacterial challenges is of importance and currently not well understood.
Methods: We used microarrays to screen ncRNA expression in the ESK-1 mouse gingival fibroblast and RAW264.7 macrophage cell lines treated with LPS from either Porphyromonas gingivalis (P. gingivalis) or Escherichia coli (E. coli). We validated the microarray analysis results through quantitative real-time PCR (qPCR) analysis of select ncRNAs.
Results: The expression of select miRNAs and lncRNAs were altered in P.Gingivalis and E.Coli cell lines following LPS treatment.
Conclusions: 1.lncRNA expression is altered in ESK-1 and RAW264.7 cells treated with LPS
2.miRNA expression is altered in ESK-1 and RAW264.7 cells treated with LPS
3.Replicate qPCR revealed statistically significant differences in ESK-1 expression of select miRNAs and lncRNAs including miR-675, miR-125a, miR-146a and lncRNA B2 SINE