IADR Abstract Archives
Lithium and Strontium-doped 58S bioactive glass for bone tissue engineering
Objectives: Bioactive glasses (BGs) are a group of surface-reactive biomaterials which extensively used in clinical applications as implants or filler materials to repair and replace diseased or damaged bone. The BGs were produced by the Sol-gel technique as a replacement for the conventional melting method. The main goal of this study is to use the potential benefits of these elements, including the effect of strontium on stimulating proliferation and alkaline phosphatase activity of MC3T3-E1, a mouse osteoblast-like cell line, in vitro as well as the antibacterial properties of lithium.
Methods: The Sr and Li-containing 58S bioactive glass with chemical composition of SiO2-CaO-P2O5-SrO-Li2O was synthesized by the Sol-Gel method. The effect of these two elements (Sr and Li) on bioactivity (ALP analysis), biocompatibility (MTT analysis), and antibacterial examinations against methicillin-resistant Staphylococcus aureus (MRSA) bacteria were performed to optimize the amount of Sr and Li. The physicochemical characterizations were carried out before and after soaking the synthesized bioactive glasses in the simulated body fluid (SBF) for different time intervals to examine the hydroxyapatite (HA), which formed on the surface of them.
Results: Structural characterization proved formation of HA on the synthesized bioactive glasses surface. The results indicated that Sr and Li had been homogeneously incorporated into bioactive glasses, having a stimulating effect on both differentiation and proliferation of MC3T3-E1 cells. The presence of Sr and Li in dissolution ions increases the mean number of DAPI-labelled nuclei which was in good agreement with live/dead assay. Additionally, the antibacterial test revealed that strontium and lithium-doped 58S bioactive glasses exhibited a potential antibacterial effect against MRSA bacteria.
Conclusions: Due to optimal proliferation and ALP activity of MC3T3-E1 cells, appropriate bioactivity and high antibacterial potential against MRSA, 58S bioactive glass with 5 mol% SrO and 5 mol% Li2O is suggested as a novel candidate for bone tissue engineering.
Methods: The Sr and Li-containing 58S bioactive glass with chemical composition of SiO2-CaO-P2O5-SrO-Li2O was synthesized by the Sol-Gel method. The effect of these two elements (Sr and Li) on bioactivity (ALP analysis), biocompatibility (MTT analysis), and antibacterial examinations against methicillin-resistant Staphylococcus aureus (MRSA) bacteria were performed to optimize the amount of Sr and Li. The physicochemical characterizations were carried out before and after soaking the synthesized bioactive glasses in the simulated body fluid (SBF) for different time intervals to examine the hydroxyapatite (HA), which formed on the surface of them.
Results: Structural characterization proved formation of HA on the synthesized bioactive glasses surface. The results indicated that Sr and Li had been homogeneously incorporated into bioactive glasses, having a stimulating effect on both differentiation and proliferation of MC3T3-E1 cells. The presence of Sr and Li in dissolution ions increases the mean number of DAPI-labelled nuclei which was in good agreement with live/dead assay. Additionally, the antibacterial test revealed that strontium and lithium-doped 58S bioactive glasses exhibited a potential antibacterial effect against MRSA bacteria.
Conclusions: Due to optimal proliferation and ALP activity of MC3T3-E1 cells, appropriate bioactivity and high antibacterial potential against MRSA, 58S bioactive glass with 5 mol% SrO and 5 mol% Li2O is suggested as a novel candidate for bone tissue engineering.