IADR Abstract Archives
Casein Phosphopeptide-Amorphous Calcium Phosphate Dentifrice: in vitro Enamel Caries
Objectives: Effects of casein phosphopeptide-amorphous calcium phosphate with sodium fluoride (CPP-ACP-F; NaF) dentifrice on in vitro enamel caries formation was compared with SnF (stannous fluoride) dentifrice, tricalcium phosphate with NaF (TCP-F) dentifrice, and CPP-ACP-F paste.
Methods: Enamel surfaces from caries-free permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. Teeth were sectioned into 5 segments, and acid-resistant varnish was placed with 2 sound enamel surface windows exposed on each segment. Each segment from a single tooth was assigned to a treatment group: 1) No Treatment Control (synthetic saliva treatment only); 2) SnF dentifrice (Sensodyne, 0.454% SnF, Glaxo-Smith-Kline); 3) TCP-F dentifrice (ClinPro 5000, 1.1% NaF, 3M ESPE); 4) CPP-ACP-F Paste (MI Paste Plus, 900 ppm NaF, 10% CPP-ACP, GC America); 5) CPP-ACP-F dentifrice (MI Paste One, 0.243% NaF, 5% KNO3, 10% CPP-ACP, GC America). Tooth segments were treated with assigned agents twice daily (180s) followed by synthetic saliva rinsing over 7d period. In vitro enamel caries were created using acidified gel (pH 4.25, 42 days). Longitudinal sections (3/tooth quarter, 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light, ANOVA, DMR).
Results: Mean lesion depths were 337±31 μm No Treatment Control; 268±27 μm SnF dentifrice; 244±23 μm TCP-F dentifrice; 173±19 μm CPP-ACP-F paste; 164±25 μm CPP-ACP-F dentifrice. All treatment agents had lesion depths significantly less than No Treatment Controls (P<.05). There was no significant difference in lesion depths between SnF and TCP-F dentifrices (P>.05). Both CPP-ACP-F paste and dentifrice provided significant decreases in lesion depth compared with SnF and TCP-F dentifrices (P<.05)
Conclusions: Dentifrices containing fluoride alone or with calcium-phosphate provided significant reductions in in vitro enamel caries lesion depths compared with synthetic saliva rinsing alone (P<.05). SnF dentifrice reduced lesion depth similar to TCP-F dentifrice (P>0.05). Greatest reductions in lesion depth were with both CPP-ACP-F paste and dentifrice compared with the other treatment groups (P<.05). Bioavailable calcium, phosphate and fluoride appear to be important in lessening in vitro caries formation, and enhancing the effects of fluoridated dentifrices.
Methods: Enamel surfaces from caries-free permanent teeth (n=10) underwent debridement and fluoride-free prophylaxis. Teeth were sectioned into 5 segments, and acid-resistant varnish was placed with 2 sound enamel surface windows exposed on each segment. Each segment from a single tooth was assigned to a treatment group: 1) No Treatment Control (synthetic saliva treatment only); 2) SnF dentifrice (Sensodyne, 0.454% SnF, Glaxo-Smith-Kline); 3) TCP-F dentifrice (ClinPro 5000, 1.1% NaF, 3M ESPE); 4) CPP-ACP-F Paste (MI Paste Plus, 900 ppm NaF, 10% CPP-ACP, GC America); 5) CPP-ACP-F dentifrice (MI Paste One, 0.243% NaF, 5% KNO3, 10% CPP-ACP, GC America). Tooth segments were treated with assigned agents twice daily (180s) followed by synthetic saliva rinsing over 7d period. In vitro enamel caries were created using acidified gel (pH 4.25, 42 days). Longitudinal sections (3/tooth quarter, 60 lesions/group) were evaluated for mean lesion depths (water imbibition, polarized light, ANOVA, DMR).
Results: Mean lesion depths were 337±31 μm No Treatment Control; 268±27 μm SnF dentifrice; 244±23 μm TCP-F dentifrice; 173±19 μm CPP-ACP-F paste; 164±25 μm CPP-ACP-F dentifrice. All treatment agents had lesion depths significantly less than No Treatment Controls (P<.05). There was no significant difference in lesion depths between SnF and TCP-F dentifrices (P>.05). Both CPP-ACP-F paste and dentifrice provided significant decreases in lesion depth compared with SnF and TCP-F dentifrices (P<.05)
Conclusions: Dentifrices containing fluoride alone or with calcium-phosphate provided significant reductions in in vitro enamel caries lesion depths compared with synthetic saliva rinsing alone (P<.05). SnF dentifrice reduced lesion depth similar to TCP-F dentifrice (P>0.05). Greatest reductions in lesion depth were with both CPP-ACP-F paste and dentifrice compared with the other treatment groups (P<.05). Bioavailable calcium, phosphate and fluoride appear to be important in lessening in vitro caries formation, and enhancing the effects of fluoridated dentifrices.