In vitro Effect of Cylindrical 3D Oriented Collagen Scaffold
Objectives: Oriented collagen is especially applicable in engineering cardiovascular tissues and nerve grafts. Laminate-typed 3D oriented collagen scaffold produced unstable performance because of its lack in strength. This study examined the effects of cylindrical 3D oriented collagen scaffold in vitro. Methods: Collagen type I of rat’s tail was prepared at a concentration of 10 mg/mL in 0.02N acetic acid. Oriented collagen substrates were produced using a hydrodynamic extrusion method. Cylindrical 3D oriented collagen scaffold was made by stuffing oriented collagen fiber in collagen tube. Cell attachment, spreading behavior, alkali phosphatase (ALP) activity were examined using bone marrow-derived osteoblasts. Results: 3D oriented collagen scaffold showed adequate strengthin the culture medium and rat blood. The number of attached cells after 24 h of observing was significantly greater on cylindrical 3D oriented collagen scaffold. The cellular spread and the expression of cytoskeletal (actin) and adhesion (vinculin) proteins were accelerated on cylindrical 3D oriented collagen scaffold. The ALP activity increased on cylindrical 3D oriented collagen scaffold at culture day 6. Conclusions: Cylindrical 3D oriented collagen scaffold increased the effects of the osteoinductivity, as represented by cell kinetics and responses. The results suggest a possible beneficial effect of vertical bone augmentation.
Division: AADR/CADR Annual Meeting
Meeting:2018 AADR/CADR Annual Meeting (Fort Lauderdale, Florida) Location: Fort Lauderdale, Florida
Year: 2018 Final Presentation ID:0646 Abstract Category|Abstract Category(s):Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
Authors
Ikeda, Takayuki
( Nihon University
, Tokyo
, Japan
)
Narita, Tatsuya
( Nihon University
, Tokyo
, Japan
)
Shioda, Yohei
( Nihon University
, Tokyo
, Japan
)
Isobe, Yoshihiro
( atree, Inc.
, Yamatokooriyama-shi
, Nara
, Japan
)
Yamaguchi, Manami
( Nihon University
, Tokyo
, Japan
)
Honda, Masaki
( Aichi-Gakuin University
, Nagoya
, Japan
)
Support Funding Agency/Grant Number: JSPS KAKENHI Grant Number JP17K11811
Financial Interest Disclosure: NONE
SESSION INFORMATION
Poster Session
Dental Materials: Biocompatibility, Bioengineering and Biologic Effects of Materials I
Thursday,
03/22/2018
, 03:45PM - 05:00PM