Objectives: To evaluate in vitro erosive effect on enamel of three brands of pre-workout (Cellucor (C4), BSN (N.O.-Xplode), MusclePharm (Assault)), and two flavors (blue raspberry (BR) and green apple (GA)).
Methods: Titratable acidities ([KOH] to neutralize products), pH, and erosion depths in enamel of each supplement were determined. The pH was measured immediately after preparing products with de-ionized water as well as after 5-, 10-, and 24 hours. Thirty extracted, caries-free molars (n=5/group) were exposed to each flavor of the pre-workout supplements for 5 days. The teeth were sectioned, and enamel erosion was measured using polarized light. A Pearson correlation test assessed the relationship between erosion depths, pH, and titratable acidities. One- and two-way ANOVA with post-hoc Tukey’s HSD test and two-sample t-test were used to detect differences between products and flavors (alpha=0.05). Results: Titratable acidities ranged from 1.93-6.46 milliliters of KOH. There was significant positive correlation between erosion and titratable acidity (r=0.82; p=0.045). Initial pH ranged from 3.7 to 4.65. There was a negative correlation between lesion depths and initial pH (r=-0.93; p=0.0078) and subsequent pH’s. A significant interaction was observed between the brands and flavors on erosion (F(2, 24)=11.10; p=0.0004). Mean lesion depth for BR was significantly greater than for GA with N.O.-Xplode (209.20±36.47 vs. 77.02±19.79; p<0.0001). For BR, the mean erosion depth for C4 was significantly greater than for N.O.-Xplode and Assault. For GA, the lesion depth of C4 was significantly greater than that of Assault, which was significantly greater than N.O.-Xplode. Conclusions: Dental erosion varies between pre-workout supplement brands and flavors