Visualization of Bacteria Using Phase-Contrast Microscope on Attitudes and Behaviors

Objectives: The purpose of this study was to determine the effect of visualization of bacteria using a phase-contrast microscope on patient attitudes and behaviors.
Methods: Upon IRB approval (2017H0274), this study utilized a randomized, evaluator-blind, controlled clinical design. Thirty participants were recruited to participate and were randomly assigned into training and control groups. All participants completed a survey of demographic information, completed a survey of oral health attitudes and behaviors, and received a comprehensive periodontal evaluation. Using a motivational interviewing framework, oral hygiene instructions were provided by one dental hygienist. The intervention consisted of the visualization of personal bacteria samples using a phase-contrast microscope. All participants received a non-definitive prophylaxis and returned in 1-month to complete the survey of oral health attitudes and behaviors and receive a periodontal evaluation.
Results: Analysis was completed on the baseline and 1-month data. Mann-Whitney U tests were used to examine the difference in oral health attitudes. No significant differences were found (p>.05) Independent sample t-tests were calculated to compare the mean clinical scores. No significant differences were found (p>.05). Chi-square tests of independence were calculated comparing the use of oral hygiene adjuncts. A significant interaction was found with the use of antibacterial rinses (χ²(1)=4.412, p<.05).
Conclusions: The visualization of bacteria with a phase contrast microscope resulted in significant increases in the use of antibacterial rinses. More research is needed to better understand the effect of the visualization of bacteria using a phase contrast microscope on improving patient attitudes and clinical outcomes.