Evaluation of a Calcium Sodium Phosphosilicate on Mineralization Using OCT
Objectives: The purpose of this study was to examine the effects of a dentifrice containing 5% calcium sodium phosphosilicate (CSP) on the remineralization of the enamel using optical coherence tomography (OCT).
Methods: Bovine incisors were sliced and shaped in a rectangular form. One group of five specimens was treated with under saturated 0.1 M lactic acid buffer solution (pH 4.75) for 10 min and then placed in artificial saliva (De group). Other specimens were stored in solutions of toothpaste containing CSP for 3 min, followed by 10-min immersion in the lactic acid buffer solution twice a day before storage in artificial saliva (CSP group). An additional group was stored in only artificial saliva (control group). From the OCT image, the peak intensity (dB) and width at (1/e2) were obtained, and the integrated value was calculated. Specimens from each group were observed using three-dimensional laser- scanning microscopy (LSM). The data for each group was subjected to one-way repeated-measures ANOVA and Tukey HSD tests (α = 0.05).
Results: There was a slight but significant increase in the integrated value observed for the control group, and a slight but significant decrease in the value observed for the De group. For the CSP group, integrated values were significant increased after seven days from the start of the experiment. LSM observation, morphologic changes including build-up of a reaction layer on the enamel surface occurred in the CSP group. In contrast, specimens in the De group showed morphologic changes such as roughening of the enamel surface and appearance of an enamel etching pattern.
Conclusions: Remineralization occurred upon immersion in the toothpaste containing CSP.