IADR Abstract Archives
RNA-seq Analysis of Different Expressions in White Sponge Nevus
Objectives: Increasing people have begun to take oral disease prevention and treatment voluntarily nowadays. However, in reality, there are some oral diseases which have not been valued by doctors and patients because of their special causes and low incidences. In this study, we explore the changes in the disease tissues, and discover the meaningful pathways and correlative networks to provide some references for future researches and treatments.
Methods: Two samples from disease area and two samples from adjacent area were collected from Shandong University, school of stomatology. Using the methods of RNA-sequencing and tissue sections, we assessed the phenotypes and mechanisms insides this disease.
Results: From the results, we identified seven single nucleotide polymorphisms(SNPs) in KRT6B, KRT14 and KRT16 genes(FIG.1A) which were related to tissue keratinization. Further, in the level of mRNA, we found that four keratinization related genes showed hundreds of folds high-expressions in diseases tissues(FIG.1B). Then in KEGG analysis, we found that many pathways showed the different expressions, and the pathways of systemic lupus erythematosus and alcoholism were highlighted which showed the highest enrichments and correlations in this disease(FIG.2). More specifically, in GO analysis, we generalized all biological processes into nine major parts such as mononuclear cell migration, cornification and angiogenesis(FIG.3A). Further studies in RNA interactions, we analyzed some major correlations between miRNA and mRNA(FIG.3B).
Conclusions: Consistent with previous study, important mutants were discovered in keratinization related genes and high expression were also found in these genes. Surprisingly, however, in the level of interactions between RNAs, we found a new unique axis from circRNAs, miRNAs and mRNAs and in the level of pathways, we found SLE-related pathways were highly expressing, and those might imply this disease was correlated with immune diversities and keratinization.
Methods: Two samples from disease area and two samples from adjacent area were collected from Shandong University, school of stomatology. Using the methods of RNA-sequencing and tissue sections, we assessed the phenotypes and mechanisms insides this disease.
Results: From the results, we identified seven single nucleotide polymorphisms(SNPs) in KRT6B, KRT14 and KRT16 genes(FIG.1A) which were related to tissue keratinization. Further, in the level of mRNA, we found that four keratinization related genes showed hundreds of folds high-expressions in diseases tissues(FIG.1B). Then in KEGG analysis, we found that many pathways showed the different expressions, and the pathways of systemic lupus erythematosus and alcoholism were highlighted which showed the highest enrichments and correlations in this disease(FIG.2). More specifically, in GO analysis, we generalized all biological processes into nine major parts such as mononuclear cell migration, cornification and angiogenesis(FIG.3A). Further studies in RNA interactions, we analyzed some major correlations between miRNA and mRNA(FIG.3B).
Conclusions: Consistent with previous study, important mutants were discovered in keratinization related genes and high expression were also found in these genes. Surprisingly, however, in the level of interactions between RNAs, we found a new unique axis from circRNAs, miRNAs and mRNAs and in the level of pathways, we found SLE-related pathways were highly expressing, and those might imply this disease was correlated with immune diversities and keratinization.
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