IADR Abstract Archives
Early Fluoride Release From 5% NaF Varnishes in vivo
Objectives: The aim of this study was to compare the early release of fluoride into unstimulated whole saliva in vivo after the application of 5%NaF, 5%NaF+TCP, and 5%NaF+ACP varnishes.
Methods: Fifteen subjects participated in a single blind, randomized, crossover design trial with a 2-week washout period between treatments. All subjects randomly received each of the three 5% NaF varnish treatments and during each treatment unstimulated whole saliva was collected at baseline, 5, 10, 20, 30, 40, 50, and 60 minutes. Approximately 0.3ml of one of the three 5% NaF varnishes was applied to the buccal surfaces of all teeth. All collected saliva samples were stored at -80°C. On the day of analysis, saliva samples were thawed to room temperature, centrifuged, and a fluoride ion specific electrode was used to measure the supernatant salivary ionic fluoride. Results were analyzed with ANOVA models followed by pairwise comparisons (5% significance level).
Results: The total amount of fluoride released during the whole hour after application was significantly higher for 5%NaF and 5%NaF+ACP. However, when the groups were compared at each time period, 5%NaF and 5%NaF+ACP released more fluoride than 5%NaF+TCP during the first 10 minutes after application, only. The three varnishes released the highest amount of fluoride during the first five minutes after application: 5%NaF 67.8 ±16.31, 5%NaF+ACP 74.41±21.53, 5%NaF+TCP 36.23±11.25 (average ppm F±SD).
Conclusions: A large amount of the fluoride released from varnishes occurs during the first few minutes after application. Varnish formulation ingredients, other than fluoride, affect the release of fluoride in vivo.
Methods: Fifteen subjects participated in a single blind, randomized, crossover design trial with a 2-week washout period between treatments. All subjects randomly received each of the three 5% NaF varnish treatments and during each treatment unstimulated whole saliva was collected at baseline, 5, 10, 20, 30, 40, 50, and 60 minutes. Approximately 0.3ml of one of the three 5% NaF varnishes was applied to the buccal surfaces of all teeth. All collected saliva samples were stored at -80°C. On the day of analysis, saliva samples were thawed to room temperature, centrifuged, and a fluoride ion specific electrode was used to measure the supernatant salivary ionic fluoride. Results were analyzed with ANOVA models followed by pairwise comparisons (5% significance level).
Results: The total amount of fluoride released during the whole hour after application was significantly higher for 5%NaF and 5%NaF+ACP. However, when the groups were compared at each time period, 5%NaF and 5%NaF+ACP released more fluoride than 5%NaF+TCP during the first 10 minutes after application, only. The three varnishes released the highest amount of fluoride during the first five minutes after application: 5%NaF 67.8 ±16.31, 5%NaF+ACP 74.41±21.53, 5%NaF+TCP 36.23±11.25 (average ppm F±SD).
Conclusions: A large amount of the fluoride released from varnishes occurs during the first few minutes after application. Varnish formulation ingredients, other than fluoride, affect the release of fluoride in vivo.