Mapping Dental Plaque Re-Accumulation: an Imaging Approach
Objectives: To evaluate a simple imaging-based approach to measuring dental plaque removal and re-accumulation after one-time use of a toothpaste or a dental gel Methods: After providing informed consent (UCI IRB 2013-9778), photographs and Plaque Index (PI)(Quigley-Hein with Turesky Modification) were recorded in 10 subjects who subsequently received oral hygiene instructions. Then, subjects brushed with a washout toothpaste (Tom’s of Maine Whole Care Toothpaste) for 1 week. Next, subjects were randomly assigned with regard to sequence of toothpaste use- beginning with either 2.6% Livionex Dental Gel (Livionex, Los Gatos, CA) or Colgate TotalR (Colgate-Palmolive, New York, NY) toothpaste. On the evening of Day 7 of week 1, subjects brushed with the first assigned toothpaste. Then they abstained from oral hygiene through the next morning, when PI was recorded, plaque was stained using plaque disclosing solution (2-Tone Disclosing Agent by Young Dental) and standardized intra-oral photographs were taken. Subsequently, subjects brushed for 2 minutes with the same toothpaste that they had used the previous night, and again PI was recorded, and plaque was stained and photographed. After 1 week washout, this was repeated with the second test toothpaste. Using image J, plaque presence expressed as percent of each tooth surface covered was computed. Results: Mean Baseline PI was 2.44 for each leg. Mean increase in Plaque Index overnight was significantly higher for Colgate (1.78) compared to Livionex (0.94) (p=0.002). After morning brushing, the reduction in the PI from Baseline also differed significantly between Colgate (0.24) and Livionex (1.13) (p=0.002). The Area covered by plaque overnight was higher for Colgate (22.3%) compared to Livionex (11.8%) (p=0.02). After morning brushing, the residual Plaque area for Colgate (9.2%) was higher than for Livionex (3.6%) p=0.039. Correlation between imaging and clinical data was 0.6953. Conclusions: A simple imaging-based approach can quantify plaque presence and removal.
Supported by: LAMMP NIH/NIBIB P41EB05890; NIH/NIBIB R03EB014852; the Arnold and Mabel Beckman Foundation; University of California SOM Seed Grant; Livionex Inc.
AADR/CADR Annual Meeting
2016 AADR/CADR Annual Meeting (Los Angeles, California) Los Angeles, California
2016 0551 Cariology Research-Microbiological Studies/Biofilm
Golabgir Anbarani, Afarin
( University of California, Irvine
, Irvine
, California
, United States
)
Vu, Thinh
( University of California, Irvine
, Irvine
, California
, United States
)
Ho, Jessica
( University of California, Irvine
, Irvine
, California
, United States
)
Forghany, Ali
( University of California, Irvine
, Irvine
, California
, United States
)
Khashai, Fatemeh
( University California Irvine
, Irvine
, California
, United States
)
Lam Ba, Tracie
( University of Calfornia, Irvine
, Irvine
, California
, United States
)
Sahni, Karan
( University of Calfornia, Irvine
, Irvine
, California
, United States
)
Wilder-smith, Petra
( University of California - Irvine
, Irvine
, California
, United States
)
NIH/NIBIB P41EB05890; NIH/NIBIB R03EB014852; the Arnold and Mabel Beckman Foundation; University of California SOM Seed Grant; Livionex Inc.
NONE