IADR Abstract Archives
Assessment of Tethered UDMA:K18 Antimicrobial Efficacy on Biofilm Activity
Objectives: The purpose of this study was to assess effect of a quaternary amine antimicrobial compound tethered to urethane dimethacrylate (UDMA-K18) versus a UDMA control.
Methods: The MTT assay was used to assess the metabolic activity of biofilms. UDMA and UDMA-K18 samples were formed in a Teflon slide and light cured on both sides (n≥21 samples/group). The slide with the samples still within the holes was placed in a closed container with a range of bacterial concentrations from 20, 10, 5, to 2.5 optical density of our multispecies (MS) or fresh full-strength saliva in BHI+1% sucrose under gentle circular agitation for 48h at 37C to allow a biofilm to develop. The samples were rinsed with sterile PBS to remove planktonic bacteria. MTT dye was added, and allowed to incubate in the dark at 37C for 4h followed by 1h incubation with SDS-DMF lysis solution. The samples were removed from the 96-well plate and absorbance of the lysis buffer was measured at 550nm.
Results: UDMA-K18 quaternary amine had significant (p≤0.01) antimicrobial activity at lower concentrations of biofilm seeding. Single species studies found that S. mutans was more susceptible to the UDMA-K18 than L. casei. The saliva derived biofilm was greatly reduced when in contact with the UDMA-K18. The figure shows the comparisons of the biofilm activity for the matched sets. Greater sensitivity to UDMA-K18 at lower biofilm seed concentrations indicates that the antimicrobial effects can be swamped by bacteria when seeded at high concentrations.
Conclusions: Since oral biofilms tend to grow from smaller concentrations of bacteria the UDMA-K18 antimicrobial material has promise for clinical use to stem the onset of biofilm on tooth surfaces.
Methods: The MTT assay was used to assess the metabolic activity of biofilms. UDMA and UDMA-K18 samples were formed in a Teflon slide and light cured on both sides (n≥21 samples/group). The slide with the samples still within the holes was placed in a closed container with a range of bacterial concentrations from 20, 10, 5, to 2.5 optical density of our multispecies (MS) or fresh full-strength saliva in BHI+1% sucrose under gentle circular agitation for 48h at 37C to allow a biofilm to develop. The samples were rinsed with sterile PBS to remove planktonic bacteria. MTT dye was added, and allowed to incubate in the dark at 37C for 4h followed by 1h incubation with SDS-DMF lysis solution. The samples were removed from the 96-well plate and absorbance of the lysis buffer was measured at 550nm.
Results: UDMA-K18 quaternary amine had significant (p≤0.01) antimicrobial activity at lower concentrations of biofilm seeding. Single species studies found that S. mutans was more susceptible to the UDMA-K18 than L. casei. The saliva derived biofilm was greatly reduced when in contact with the UDMA-K18. The figure shows the comparisons of the biofilm activity for the matched sets. Greater sensitivity to UDMA-K18 at lower biofilm seed concentrations indicates that the antimicrobial effects can be swamped by bacteria when seeded at high concentrations.
Conclusions: Since oral biofilms tend to grow from smaller concentrations of bacteria the UDMA-K18 antimicrobial material has promise for clinical use to stem the onset of biofilm on tooth surfaces.
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