IADR Abstract Archives

Mechanical stability of collagen membranes: an in vitro study

Objectives: For large horizontal augmentations or cases requiring even a minor vertical augmentation there is a clinical need for mechanically stable resorbable membranes that can be fixated with pins to immobilize the graft material. An investigation of native non-chemically cross-linked collagen membranes focusing on differences in degradation behavior and tissue integration has indicated good mechanical properties of a newly developed collagen membrane (CX). The aim of this study was to compare the mechanical strength of commonly used native non-chemically cross-linked [NXL] and chemically cross-linked [XL] collagen membranes in-vitro.
Methods: Five NXL* and five XL§ membranes were included (n=6 samples each) and immersed in saline at room temperature until fully hydrated. Force at break (N), stress at break (N/mm2) and suture retention (N) were tested in-vitro using a Zwick/Roell Z2.5 tensile tester (Ulm, Germany). Statistical analysis of in vitro test results was performed using Wilcoxon test with Bonferroni corrections for multiple comparisons. CX was the comparison group for all statistical tests. Significance was set at (P< 0.05).
Results: The highest median force at break 21.2 N (IQR: 13-23.7), stress at break 14.2 N/mm2 (IQR: 9.1-16.3) and suture retention 6.1 N (IQR: 5.9-6.5) was measured for CX. Results were significant compared with BG, JS, OF and BM, CY & OP membranes for force and stress at wet break (Table). Suture retention reached significance compared to all but one membrane. No trends in the mechanical strength performance across the two groups, NXL and XL, were observed.
Conclusions: After wetting the CX membrane has higher mechanical strength, resists higher stress and has higher pull out force than all other included NXL and XL collagen membranes. The CX membrane provides high strength required for use as a barrier membrane for GBR procedures to treat large defects such as horizontal ridge augmentation.
AADR/CADR Annual Meeting
2016 AADR/CADR Annual Meeting (Los Angeles, California)
Los Angeles, California
2016
1683
  • Gasser, Angelines  ( Nobel Biocare Services AG , Kloten Kloten , Switzerland )
  • Wessing, Bastian  ( Luisenhospital Aachen , Aachen Aachen , Nordrhein-Westfalen , Germany )
  • Eummelen, Lizette  ( Matricel GmbH , Herzogenrath Herzogenrath , Germany )
  • Büren, Anne  ( Matricel GmbH , Herzogenrath Herzogenrath , Germany )
  • Leemhuis, Hans  ( Matricel GmbH , Herzogenrath Herzogenrath , Germany )
  • Jointly sponsored by Nobel Biocare Services AG, Kloten, Switzerland and Matricel GmbH, Herzogenrath, Germany
    Angelines Gasser is an employee of Nobel Biocare Services AG. Bastian Wessing has no financial interest to disclose. Lizette Eummelen, Anne Bühren and Hans Leemhuis are employees of Matricel GmbH.
    Poster Session
    Implantology Research VI
    Saturday, 03/19/2016 , 10:45AM - 12:00PM
    Mechanical strength test results
    Collagen
    Membrane
    Force at break [N]
    median (IQR)
    p- valueStress at break [N/mm2]
    median (IQR)
    p- valueSuture retention [N]
    Median (IQR)
    p- value
    CX21.2 (13-23.7) 14.2 (9.1-16.3) 6.1 (5.9-6.5) 
    BE11.2 (7.2-14.0)ns5.1 (3.9-7.1)ns2.8 (2.7-3.2)0.045
    BG3.1 (2.2-4.2)0.0181.3 (0.8-1.6)0.0181.5 (1.4-1.8)0.045
    BM5.4 (5.0-6.9)0.0183.6 (3.2-3.9)0.0182.3 (1.8-2.8)0.018
    CO15.6 (15.5-.1)ns8.7 (6.8-9.6)ns4.3 (3.5-5.0)0.018
    CY3.0 (2.9-3.7)0.0181.6 (1.4-1.9)0.0181.4 (1.4-1.6)0.018
    JS5.2 (3.8-6.9)0.0363.6 (2.6-4.1)0.0362.1 (1.9-2.3)0.036
    ML9.3 (6.3-2.0)ns3.4 (2.5-4.7)0.0182.0 (1.9-2.2)0.018
    OF3.6 (2.5-4.6)0.0181.3 (0.8-1.7)0.0182.9 (2.6-3.9)ns
    OP5.7 (4.9-6.6)0.0184.1 (3.4-5.4)0.0180.1 (0.1-0.1)0.045
           
    IQR: Inter-quartile range (Q1-Q3); ns: not significant p>0.05. *NXL: creos xenoprotect [CX] (Nobel Biocare), Bio-Gide [BG] (Geistlich), Copios [CO] (Zimmer), Osseoguard Flex [OF] (3i), Jason [JS] (botiss) §XL: BioMend [BM] & Biomend Extend [BE] (Zimmer), Cytoplast RTM [CY] (Osteogenix), Mem-Lok [ML] (BioHorizons), OssixPlus [OP] (Datum Dental Ltd)